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Diagnostic microbiologic methods in the GEMS-1 case/control study.
- Source :
-
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America [Clin Infect Dis] 2012 Dec; Vol. 55 Suppl 4, pp. S294-302. - Publication Year :
- 2012
-
Abstract
- To understand the etiology of moderate-to-severe diarrhea among children in high mortality areas of sub-Saharan Africa and South Asia, we performed a comprehensive case/control study of children aged <5 years at 7 sites. Each site employed an identical case/control study design and each utilized a uniform comprehensive set of microbiological assays to identify the likely bacterial, viral and protozoal etiologies. The selected assays effected a balanced consideration of cost, robustness and performance, and all assays were performed at the study sites. Identification of bacterial pathogens employed streamlined conventional bacteriologic biochemical and serological algorithms. Diarrheagenic Escherichia coli were identified by application of a multiplex polymerase chain reaction assay for enterotoxigenic, enteroaggregative, and enteropathogenic E. coli. Rotavirus, adenovirus, Entamoeba histolytica, Giardia enterica, and Cryptosporidium species were detected by commercially available enzyme immunoassays on stool samples. Samples positive for adenovirus were further evaluated for adenovirus serotypes 40 and 41. We developed a novel multiplex assay to detect norovirus (types 1 and 2), astrovirus, and sapovirus. The portfolio of diagnostic assays used in the GEMS study can be broadly applied in developing countries seeking robust cost-effective methods for enteric pathogen detection.
- Subjects :
- Africa South of the Sahara
Asia, Western
Case-Control Studies
Cryptosporidium isolation & purification
Diarrhea etiology
Diarrhea virology
Entamoeba histolytica isolation & purification
Escherichia coli isolation & purification
Giardia isolation & purification
Humans
Immunoenzyme Techniques
Microbiological Techniques methods
Multicenter Studies as Topic methods
Parasitology methods
Polymerase Chain Reaction
Quality Assurance, Health Care
Quality Control
Virology methods
Viruses isolation & purification
Diarrhea microbiology
Diarrhea parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6591
- Volume :
- 55 Suppl 4
- Database :
- MEDLINE
- Journal :
- Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
- Publication Type :
- Academic Journal
- Accession number :
- 23169941
- Full Text :
- https://doi.org/10.1093/cid/cis754