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Molecular characterization of extended-spectrum-beta-lactamase-producing Escherichia coli isolates from red foxes in Portugal.
- Source :
-
Archives of microbiology [Arch Microbiol] 2013 Feb; Vol. 195 (2), pp. 141-4. Date of Electronic Publication: 2012 Nov 22. - Publication Year :
- 2013
-
Abstract
- The presence of broad-spectrum-cephalosporin-resistant Escherichia coli isolates and the implicated mechanisms of resistance and virulence factor genes were investigated in red fox (Vulpes vulpes) in Portugal. Cefotaxime-resistant E. coli isolates were isolated from two of 52 fecal samples (4 %), being both ESBL producers. The β-lactamase genes found in the two isolates were bla(SHV-12) + bla(TEM-1b). The tet(A) and sul2 genes were also detected in these isolates, together with the non-classical class 1 integron (intI1-dfrA12-orfF-aadA2-cmlA1-aadA1-qacH-IS440-sul3) with the PcH1 promoter. The two isolates belonged to the phylogroup A. Amino acid changes in GyrA (S83L + D87G) and ParC (S80I) proteins were identified in our study. Concerning MLST typing, both isolates were assigned to ST1086, never found before in wild animals, and they presented closely related PFGE patterns. This study reveals the presence of ESBL-producing E. coli isolates, in a wild ecosystem, which could be disseminated through the environment to other niches.
- Subjects :
- Animals
Anti-Bacterial Agents pharmacology
Bacterial Typing Techniques
Cephalosporins pharmacology
Electrophoresis, Gel, Pulsed-Field
Escherichia coli enzymology
Escherichia coli genetics
Escherichia coli isolation & purification
Feces microbiology
Integrons
Microbial Sensitivity Tests
Multilocus Sequence Typing
Phylogeny
Portugal
Promoter Regions, Genetic
Cephalosporin Resistance
Escherichia coli classification
Foxes microbiology
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-072X
- Volume :
- 195
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Archives of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 23179656
- Full Text :
- https://doi.org/10.1007/s00203-012-0853-7