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Protein tyrosine phosphatase µ (PTP µ or PTPRM), a negative regulator of proliferation and invasion of breast cancer cells, is associated with disease prognosis.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (11), pp. e50183. Date of Electronic Publication: 2012 Nov 20. - Publication Year :
- 2012
-
Abstract
- Background: PTPRM has been shown to exhibit homophilic binding and confer cell-cell adhesion in cells including epithelial and cancer cells. The present study investigated the expression of PTPRM in breast cancer and the biological impact of PTPRM on breast cancer cells.<br />Design: Expression of PTPRM protein and gene transcript was examined in a cohort of breast cancer patients. Knockdown of PTPRM in breast cancer cells was performed using a specific anti-PTPRM transgene. The impact of PTPRM knockdown on breast cancer was evaluated using in vitro cell models.<br />Results: A significant decrease of PTPRM transcripts was seen in poorly differentiated and moderately differentiated tumours compared with well differentiated tumours. Patients with lower expression of PTPRM had shorter survival compared with those which had a higher level of PTPRM expression. Knockdown of PTPRM increased proliferation, adhesion, invasion and migration of breast cancer cells. Furthermore, knockdown of PTPRM in MDA-MB-231 cells resulted in increased cell migration and invasion via regulation of the tyrosine phosphorylation of ERK and JNK.<br />Conclusions: Decreased expression of PTPRM in breast cancer is correlated with poor prognosis and inversely correlated with disease free survival. PTPRM coordinated cell migration and invasion through the regulation of tyrosine phosphorylation of ERK and JNK.
- Subjects :
- Breast Neoplasms metabolism
Breast Neoplasms mortality
Breast Neoplasms pathology
Cell Adhesion
Cell Line, Tumor
Cell Movement
Cell Proliferation
Extracellular Signal-Regulated MAP Kinases metabolism
Female
Gene Knockdown Techniques
Humans
MAP Kinase Kinase 4 metabolism
Neoplasm Grading
Phosphorylation
Prognosis
RNA, Messenger metabolism
Receptor-Like Protein Tyrosine Phosphatases, Class 2 antagonists & inhibitors
Receptor-Like Protein Tyrosine Phosphatases, Class 2 metabolism
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Survival Analysis
Breast Neoplasms genetics
Extracellular Signal-Regulated MAP Kinases genetics
Gene Expression Regulation, Neoplastic
MAP Kinase Kinase 4 genetics
Receptor-Like Protein Tyrosine Phosphatases, Class 2 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23185569
- Full Text :
- https://doi.org/10.1371/journal.pone.0050183