Back to Search
Start Over
Oocyte extract improves epigenetic reprogramming of yak fibroblast cells and cloned embryo development.
- Source :
-
Theriogenology [Theriogenology] 2013 Feb; Vol. 79 (3), pp. 462-9. Date of Electronic Publication: 2012 Nov 24. - Publication Year :
- 2013
-
Abstract
- The objective was to investigate the effects of bovine oocyte extract (BOE) on epigenetic reprogramming of yak fibroblast cells, based on their cell cycle status, histone acetylation, DNA methylation, gene expression, and cloned blastocyst formation. Permeabilization of yak fibroblasts after treatment with 10 or 50 μL of BOE (treated-S and treated-L groups, respectively) for 24 hours increased (P < 0.05) the cell population at the G(0)/G(1) phase (85.2 ± 2.3% and 89.6 ± 1.5%, respectively) compared with controls (75.4 ± 1.1%). Acetylation at lysine 9 of histone H3 was also higher (26.1 ± 1.4 and 33.5 ± 2.1) than in the control group (15.3 ± 1.6; P < 0.05). Moreover, BOE reduced methylation of the promoter regions of Oct-4 and Nanog (76.4% and 72.2%; and 35.6% and 30.0%, respectively) compared with the control group (92.1% and 47.8%; P < 0.05). In addition, the relative expression levels of HDAC-1, HADC-2, Dnmt-1, and Dnmt-3a were downregulated (P < 0.05) after yak fibroblasts were treated with BOE. Furthermore, when yak fibroblasts were used for interspecies somatic cell nuclear transfer after BOE treatment, 8-cell and blastocyst formation rates significantly exceeded those of the control. In conclusion, BOE induced epigenetic reprogramming of yak fibroblasts, making them suitable donors for yak interspecies somatic cell nuclear transfer.<br /> (Crown Copyright © 2013. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Acetylation
Animals
Blastocyst physiology
Cell Membrane Permeability
Cloning, Organism methods
DNA Methylation genetics
Embryonic Development physiology
Female
Gene Expression
Histone Deacetylases genetics
Histones metabolism
Nuclear Transfer Techniques veterinary
Cattle
Cell Differentiation physiology
Cloning, Organism veterinary
Epigenesis, Genetic physiology
Fibroblasts physiology
Oocytes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3231
- Volume :
- 79
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 23186945
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2012.10.019