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Association between magnitude of the virus-specific plasmablast response and disease severity in dengue patients.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2013 Jan 01; Vol. 190 (1), pp. 80-7. Date of Electronic Publication: 2012 Nov 30. - Publication Year :
- 2013
-
Abstract
- Dengue is a globally expanding disease caused by infection with dengue virus (DENV) that ranges from febrile illness to acute disease with serious complications. Secondary infection predisposes individuals to more severe disease, and B lymphocytes may play a role in this phenomenon through production of Ab that enhance infection. To better define the acute B cell response during dengue, we analyzed peripheral B cells from an adult Brazilian hospital cohort with primary and secondary DENV infections of varying clinical severity. Circulating B cells in dengue patients were proliferating, activated, and apoptotic relative to individuals with other febrile illnesses. Severe secondary DENV infection was associated with extraordinary peak plasmablast frequencies between 4 and 7 d of illness, averaging 46% and reaching 87% of B cells, significantly greater than those seen in mild illness or primary infections. On average >70% of IgG-secreting cells in individuals with severe secondary DENV infection were DENV specific. Plasmablasts produced Ab that cross-reacted with heterotypic DENV serotypes, but with a 3-fold greater reactivity to DENV-3, the infecting serotype. Plasmablast frequency did not correlate with acute serum-neutralizing Ab titers to any DENV serotype regardless of severity of disease. These findings indicate that massive expansion of DENV-specific and serotype cross-reactive plasmablasts occurs in acute secondary DENV infection of adults in Brazil, which is associated with increasing disease severity.
- Subjects :
- Acute Disease
Adolescent
Adult
Aged
B-Lymphocyte Subsets immunology
B-Lymphocyte Subsets pathology
B-Lymphocyte Subsets virology
Brazil
Child
Cohort Studies
Dengue immunology
Dengue Virus pathogenicity
Female
Humans
Male
Middle Aged
Plasma Cells pathology
T-Lymphocyte Subsets immunology
T-Lymphocyte Subsets pathology
T-Lymphocyte Subsets virology
Young Adult
Dengue pathology
Dengue virology
Dengue Virus immunology
Plasma Cells immunology
Plasma Cells virology
Severity of Illness Index
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 190
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 23203929
- Full Text :
- https://doi.org/10.4049/jimmunol.1103350