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[Construction of stable focal adhesion kinase knockdown cell line and preliminary study of its properties].
- Source :
-
Yao xue xue bao = Acta pharmaceutica Sinica [Yao Xue Xue Bao] 2012 Sep; Vol. 47 (9), pp. 1128-33. - Publication Year :
- 2012
-
Abstract
- Malignant melanoma still remains to be a serious health threat. Overexpression of focal adhesion kinase (FAK) in melanoma has suggested that FAK could be a promising target for therapeutic intervention. To further investigate the function of FAK in melanoma, FAK expression was down-regulated by stable transfection of plasmid harboring FAK small interfering RNA (siRNA) into melanoma cell line. Two stable cell lines, F10-siFAK and F10-control, have been constructed and screened. Compared with the F10-control, both the mRNA and protein levels of FAK decreased significantly, and the cell cycle of F10-siFAK was arrested at G1 phase. Furthermore, the tumor growth rate of F10-siFAK cells was notably slower than that of F10-control in in vivo tumor models. These results show that FAK is an important regulatory gene in melanoma. The stable FAK-knockdown melanoma cell line is an useful tool for further investigation of FAK's function in the progression of melanoma, and also an effective means of drug screening for anti-melanoma therapeutics.
- Subjects :
- Animals
Cell Cycle
Cell Line, Tumor
Cell Proliferation
Down-Regulation
G1 Phase
Gene Knockdown Techniques
Mice
Mice, Inbred C57BL
Mitogen-Activated Protein Kinase 1 metabolism
Mitogen-Activated Protein Kinase 3 metabolism
Plasmids
RNA, Messenger metabolism
Transfection
Focal Adhesion Protein-Tyrosine Kinases genetics
Focal Adhesion Protein-Tyrosine Kinases metabolism
Melanoma, Experimental enzymology
Melanoma, Experimental pathology
RNA, Small Interfering genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0513-4870
- Volume :
- 47
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Yao xue xue bao = Acta pharmaceutica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 23227540