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Pulp mill wastewater sediment reveals novel methanogenic and cellulolytic populations.
- Source :
-
Water research [Water Res] 2013 Feb 01; Vol. 47 (2), pp. 683-92. Date of Electronic Publication: 2012 Nov 21. - Publication Year :
- 2013
-
Abstract
- Pulp mill wastewater generated from wheat straw is characterized as high alkalinity and very high COD pollution load. A naturally developed microbial community in a pulp mill wastewater storage pool that had been disused were investigated in this study. Owing to natural evaporation and a huge amount of lignocellulose's deposition, the wastewater sediment contains high concentrations of organic matters and sodium ions, but low concentrations of chloride and carbonate. The microbiota inhabiting especially anaerobic community, including methanogenic arhcaea and cellulolytic species, was studied. All archaeal sequences fall into 2 clusters of family Halobacteriaceae and methanogenic archaeon in the phylum Euryarchaeota. In the methanogenic community, phylogenetic analysis of methyl coenzyme M reductase A (mcrA) genes targeted to novel species in genus Methanoculleus or novel genus of order Methanomicrobiales. The predominance of Methanomicrobiales suggests that methanogenesis in this system might be driven by the hydrogenotrophic pathway. As the important primary fermenter for methane production, the cellulolytic community of enzyme GHF48 was found to be dominated by narrower breadth of novel clostridial cellulase genes. Novel anoxic functional members in such extreme sediment provide the possibility of enhancing the efficiency of anoxic treatment of saline and alkaline wastewaters, as well as benefiting to the biomass transformation and biofuel production processes.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Archaeal Proteins genetics
Archaeal Proteins metabolism
Cellulases genetics
Cellulases metabolism
China
Clostridium classification
Clostridium isolation & purification
Clostridium metabolism
Euryarchaeota classification
Euryarchaeota isolation & purification
Euryarchaeota metabolism
Gene Expression Regulation, Archaeal
Halobacteriaceae classification
Halobacteriaceae isolation & purification
Halobacteriaceae metabolism
Hydrogen metabolism
Hydrolysis
Industry
Methanomicrobiaceae classification
Methanomicrobiaceae isolation & purification
Methanomicrobiaceae metabolism
Microbial Viability
Molecular Typing
Oxidoreductases genetics
Oxidoreductases metabolism
Paper
Phylogeny
Wastewater microbiology
Wood chemistry
Wood microbiology
Wood parasitology
Cellulose metabolism
Clostridium growth & development
Euryarchaeota growth & development
Halobacteriaceae growth & development
Methane metabolism
Methanomicrobiaceae growth & development
Wastewater parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-2448
- Volume :
- 47
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Water research
- Publication Type :
- Academic Journal
- Accession number :
- 23228889
- Full Text :
- https://doi.org/10.1016/j.watres.2012.10.038