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Different susceptibilities of Leishmania spp. promastigotes to the Annona muricata acetogenins annonacinone and corossolone, and the Platymiscium floribundum coumarin scoparone.
- Source :
-
Experimental parasitology [Exp Parasitol] 2013 Mar; Vol. 133 (3), pp. 334-8. Date of Electronic Publication: 2012 Dec 08. - Publication Year :
- 2013
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Abstract
- Leishmaniasis is a zoonotic disease that can manifest itself in visceral and cutaneous form. The aim of this study was to search for new leishmanicidal compounds. Preliminarily, Artemia salina assay was applied to compounds from two plants found in Northeastern Brazil, Platymiscium floribundum and Annona muricata. Then these compounds were tested against three Leishmania species (Leishmania donovani, Leishmania mexicana and Leishmania major). A screening assay using luciferase-expressing promastigote form were used to measure the viability of promastigote One coumarin, scoparone, isolated from P. floribundum and two acetogenins, annonacinone and corossolone isolated from A. muricata showed leishmanicidal activity in all species tested. Nevertheless, Leishmania species indicated different susceptibilities in relation to the tested compounds: L. mexicana was more sensitive to scoparone followed by L. major and L. donovani. The three species presented similar inhibition to corossolone and annonacinone. Acetogenin annonacinone (EC(50)=6.72-8.00 μg/mL) indicated high leishmanicidal activity; corossolone (EC(50)=16.14-18.73 μg/mL) and scoparone (EC(50)=9.11-27.51 μg/mL) moderate activity. A. saline larvae were less sensitive to the coumarin scoparone and acetogenin corossolone was the most toxic. In conclusion, the leishmanicidal activity demonstrated by the coumarin and acetogenins indicate these compounds for further studies aiming the development of new leishmanicidal agents.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- 4-Butyrolactone analogs & derivatives
4-Butyrolactone isolation & purification
4-Butyrolactone pharmacology
4-Butyrolactone toxicity
Animals
Antiprotozoal Agents toxicity
Artemia drug effects
Biological Assay
Brazil
Chromatography, Thin Layer
Coumarins isolation & purification
Coumarins pharmacology
Coumarins toxicity
Dose-Response Relationship, Drug
Furans isolation & purification
Furans pharmacology
Furans toxicity
Plant Extracts chemistry
Plant Extracts toxicity
Annona chemistry
Antiprotozoal Agents pharmacology
Fabaceae chemistry
Leishmania drug effects
Plant Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 133
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 23232251
- Full Text :
- https://doi.org/10.1016/j.exppara.2012.11.025