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Regulation of microtubule stability and organization by mammalian Par3 in specifying neuronal polarity.
- Source :
-
Developmental cell [Dev Cell] 2013 Jan 14; Vol. 24 (1), pp. 26-40. Date of Electronic Publication: 2012 Dec 27. - Publication Year :
- 2013
-
Abstract
- Polarization of mammalian neurons with a specified axon requires precise regulation of microtubule and actin dynamics in the developing neurites. Here we show that mammalian partition defective 3 (mPar3), a key component of the Par polarity complex that regulates the polarization of many cell types including neurons, directly regulates microtubule stability and organization. The N-terminal portion of mPar3 exhibits strong microtubule binding, bundling, and stabilization activity, which can be suppressed by its C-terminal portion via an intramolecular interaction. Interestingly, the intermolecular oligomerization of mPar3 is able to relieve the intramolecular interaction and thereby promote microtubule bundling and stabilization. Furthermore, disruption of this microtubule regulatory activity of mPar3 impairs its function in axon specification. Together, these results demonstrate a role for mPar3 in directly regulating microtubule organization that is crucial for neuronal polarization.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Subjects :
- Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Axons metabolism
COS Cells
Cell Adhesion Molecules antagonists & inhibitors
Cell Adhesion Molecules genetics
Cell Cycle Proteins
Cells, Cultured
Cytoskeleton metabolism
Fluorescence Resonance Energy Transfer
Green Fluorescent Proteins
Hippocampus cytology
Hippocampus metabolism
Immunoprecipitation
Mice
Microtubules chemistry
Molecular Sequence Data
Neurons metabolism
Protein Structure, Tertiary
RNA, Small Interfering genetics
Sequence Homology, Amino Acid
Signal Transduction
Cell Adhesion Molecules metabolism
Cell Polarity
Microtubules physiology
Neurogenesis physiology
Neurons cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1551
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Developmental cell
- Publication Type :
- Academic Journal
- Accession number :
- 23273878
- Full Text :
- https://doi.org/10.1016/j.devcel.2012.11.014