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Amplification of complete gag gene sequences from geographically distinct equine infectious anemia virus isolates.
- Source :
-
Journal of virological methods [J Virol Methods] 2013 Apr; Vol. 189 (1), pp. 41-6. Date of Electronic Publication: 2013 Jan 11. - Publication Year :
- 2013
-
Abstract
- In the current study, primers described previously and modified versions of these primers were evaluated for amplification of full-length gag genes from different equine infectious anemia virus (EIAV) strains from several countries, including the USA, Germany and Japan. Each strain was inoculated into a primary horse leukocyte culture, and the full-length gag gene was amplified by reverse transcription polymerase chain reaction. Each amplified gag gene was cloned into a plasmid vector for sequencing, and the detectable copy numbers of target DNA were determined. Use of a mixture of two forward primers and one reverse primer in the polymerase chain reaction enabled the amplification of all EIAV strains used in this study. However, further study is required to confirm these primers as universal for all EIAV strains. The nucleotide sequence of gag is considered highly conserved, as evidenced by the use of gag-encoded capsid proteins as a common antigen for the detection of EIAV in serological tests. However, significant sequence variation in the gag genes of different EIAV strains was found in the current study.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Base Sequence
Cells, Cultured
Equine Infectious Anemia virology
Gene Amplification
Genetic Variation
Horses
Molecular Sequence Data
Sequence Alignment
Sequence Analysis, DNA
Equine Infectious Anemia diagnosis
Genes, gag
Infectious Anemia Virus, Equine genetics
Reverse Transcriptase Polymerase Chain Reaction veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0984
- Volume :
- 189
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 23318370
- Full Text :
- https://doi.org/10.1016/j.jviromet.2012.12.010