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Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings.
- Source :
-
Plant molecular biology [Plant Mol Biol] 2013 Mar; Vol. 81 (4-5), pp. 347-61. Date of Electronic Publication: 2013 Jan 18. - Publication Year :
- 2013
-
Abstract
- Germination followed by seedling growth constitutes two essential steps in the initiation of a new life cycle in plants, and in cereals, completion of these steps is regulated by sugar starvation and the hormone gibberellin. A calcium-dependent protein kinase 1 gene (OsCDPK1) was identified by differential screening of a cDNA library derived from sucrose-starved rice suspension cells. The expression of OsCDPK1 was found to be specifically activated by sucrose starvation among several stress conditions tested as well as activated transiently during post-germination seedling growth. In gain- and loss-of-function studies performed with transgenic rice overexpressing a constitutively active or RNA interference gene knockdown construct, respectively, OsCDPK1 was found to negatively regulate the expression of enzymes essential for GA biosynthesis. In contrast, OsCDPK1 activated the expression of a 14-3-3 protein, GF14c. Overexpression of either constitutively active OsCDPK1 or GF14c enhanced drought tolerance in transgenic rice seedlings. Hence, our studies demonstrated that OsCDPK1 transduces the post-germination Ca(2+) signal derived from sugar starvation and GA, refines the endogenous GA concentration and prevents drought stress injury, all essential functions to seedling development at the beginning of the life cycle in rice.
- Subjects :
- Droughts
Enzyme Induction drug effects
Feedback, Physiological drug effects
Gene Expression Regulation, Developmental drug effects
Gene Expression Regulation, Plant drug effects
Gibberellins pharmacology
Homozygote
Models, Biological
Organ Size drug effects
Oryza drug effects
Oryza genetics
Oryza growth & development
Plant Proteins metabolism
Plants, Genetically Modified
Protein Kinases genetics
Seedlings anatomy & histology
Seedlings drug effects
Seedlings genetics
Up-Regulation drug effects
14-3-3 Proteins metabolism
Adaptation, Physiological drug effects
Adaptation, Physiological genetics
Carbohydrates deficiency
Gibberellins biosynthesis
Oryza enzymology
Protein Kinases biosynthesis
Seedlings physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-5028
- Volume :
- 81
- Issue :
- 4-5
- Database :
- MEDLINE
- Journal :
- Plant molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 23329372
- Full Text :
- https://doi.org/10.1007/s11103-012-0006-z