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SDR9C7 promotes lymph node metastases in patients with esophageal squamous cell carcinoma.
- Source :
-
PloS one [PLoS One] 2013; Vol. 8 (1), pp. e52184. Date of Electronic Publication: 2013 Jan 14. - Publication Year :
- 2013
-
Abstract
- Background: The major reason for the poor prognosis of esophageal squamous cell carcinoma (ESCC) patients is lymph node (LN) metastases.<br />Methodology/principal: In the present study, gene expression profiling assay (GEP) was performed to identify the differences in gene expression profiles between primary ESCC tumors that were with LN metastases (N(+)) and those without LN metastases (N(-)).<br />Conclusions/significance: A total of 23 genes were identified as being significantly elevated, and 30 genes were sharply decreased in ESCC tumors that were N(+) compared with N- tumors. Among these genes, two transcripts of the short chain dehydrogenase/reductase family 9C, member 7 (SDR9C7) were observed 7 times more frequently in N(+) compared with N(-) tumors. Immunohistochemical staining showed that SDR9C7 expression closely correlated with metastasis, and would be a prognostic marker for ESCC patients. To investigate the role of SDR9C7 in the ESCC metastasis, repeated transwell assays were adopted to establish highly and non-invasive ESCC sublines, and western blot showed that SDR9C7 expression was markedly higher in highly invasive cells compared with non-invasive ones. Down-regulation of SDR9C7 dramatically inhibited the metastatic abilities in vitro and in vivo, and repressed the expression of MMP11 in highly invasive cells, indicating that SDR9C7 promotes ESCC metastasis partly through regulation of MMP11, and might be a potential prognostic and therapeutic marker for ESCC patients.
- Subjects :
- Adult
Aged
Animals
Blotting, Western
Carcinoma, Squamous Cell genetics
Cell Line, Tumor
Down-Regulation genetics
Esophageal Neoplasms genetics
Esophageal Squamous Cell Carcinoma
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Humans
Immunohistochemistry
Lentivirus metabolism
Lymphatic Metastasis genetics
Male
Mice
Middle Aged
Multivariate Analysis
Neoplasm Invasiveness
Oxidoreductases genetics
Prognosis
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Small Interfering metabolism
Up-Regulation genetics
Carcinoma, Squamous Cell enzymology
Carcinoma, Squamous Cell pathology
Esophageal Neoplasms enzymology
Esophageal Neoplasms pathology
Lymphatic Metastasis pathology
Oxidoreductases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23341893
- Full Text :
- https://doi.org/10.1371/journal.pone.0052184