Back to Search
Start Over
Variable EBV DNA load distributions and heterogeneous EBV mRNA expression patterns in the circulation of solid organ versus stem cell transplant recipients.
- Source :
-
Clinical & developmental immunology [Clin Dev Immunol] 2012; Vol. 2012, pp. 543085. Date of Electronic Publication: 2012 Dec 30. - Publication Year :
- 2012
-
Abstract
- Unlabelled: Epstein-Barr virus (EBV) driven post-transplant lymphoproliferative disease (PTLD) is a heterogeneous and potentially life-threatening condition. Early identification of aberrant EBV activity may prevent progression to B-cell lymphoma. We measured EBV DNA load and RNA profiles in plasma and cellular blood compartments of stem cell transplant (SCT; n = 5), solid organ transplant recipients (SOT; n = 15), and SOT having chronic elevated EBV-DNA load (n = 12). In SCT, EBV DNA was heterogeneously distributed, either in plasma or leukocytes or both. In SOT, EBV DNA load was always cell associated, predominantly in B cells, but occasionally in T cells (CD4 and CD8) or monocytes. All SCT with cell-associated EBV DNA showed BARTs and EBNA1 expression, while LMP1 and LMP2 mRNA was found in 1 and 3 cases, respectively. In SOT, expression of BARTs was detected in all leukocyte samples. LMP2 and EBNA1 mRNA was found in 5/15 and 2/15, respectively, but LMP1 mRNA in only 1, coinciding with severe PTLD and high EBV DNA.<br />Conclusion: EBV DNA is differently distributed between white cells and plasma in SOT versus SCT. EBV RNA profiling in blood is feasible and may have added value for understanding pathogenic virus activity in patients with elevated EBV-DNA.
- Subjects :
- Adolescent
Adult
B-Lymphocytes immunology
B-Lymphocytes virology
Child
DNA, Viral blood
DNA, Viral immunology
Epstein-Barr Virus Infections blood
Epstein-Barr Virus Infections immunology
Epstein-Barr Virus Nuclear Antigens genetics
Epstein-Barr Virus Nuclear Antigens immunology
Female
Herpesvirus 4, Human immunology
Humans
Leukocytes immunology
Leukocytes virology
Lymphoproliferative Disorders blood
Lymphoproliferative Disorders immunology
Lymphoproliferative Disorders virology
Male
Middle Aged
Monocytes immunology
Monocytes virology
RNA, Messenger biosynthesis
RNA, Messenger immunology
RNA, Viral biosynthesis
RNA, Viral immunology
T-Lymphocytes immunology
T-Lymphocytes virology
Viral Load
Viral Matrix Proteins genetics
Viral Matrix Proteins immunology
Viral Proteins genetics
Viral Proteins immunology
Young Adult
DNA, Viral genetics
Epstein-Barr Virus Infections virology
Herpesvirus 4, Human genetics
RNA, Messenger genetics
RNA, Viral genetics
Stem Cell Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1740-2530
- Volume :
- 2012
- Database :
- MEDLINE
- Journal :
- Clinical & developmental immunology
- Publication Type :
- Academic Journal
- Accession number :
- 23346186
- Full Text :
- https://doi.org/10.1155/2012/543085