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Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.

Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.

Authors :
Morimoto S
Tomohiro T
Maruyama N
Hatanaka Y
Source :
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2013 Mar 04; Vol. 49 (18), pp. 1811-3. Date of Electronic Publication: 2013 Jan 25.
Publication Year :
2013

Abstract

A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

Details

Language :
English
ISSN :
1364-548X
Volume :
49
Issue :
18
Database :
MEDLINE
Journal :
Chemical communications (Cambridge, England)
Publication Type :
Academic Journal
Accession number :
23349004
Full Text :
https://doi.org/10.1039/c3cc38594a