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[Protective effect of propofol and resveratrol pretreatment against hepatocyte apoptosis in rats with hepatic ischemia-reperfusion injury].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2013 Jan; Vol. 33 (1), pp. 80-5. - Publication Year :
- 2013
-
Abstract
- Objective: To observe the effect of resveratrol and propofol, used either alone or in combination, on hepatocyte apoptosis in rats with hepatic ischemia-reperfusion injury (HIRI).<br />Methods: A total of 144 male SD rats were randomized into 8 equal groups, including a sham-operated group and 7 HIRI (established using Pringle method) groups with pretreatments with normal saline, Tween80, propofol (10 or 20 mg·kg(-1)·h(-1)), or resveratrol (10 or 20 mg/kg), or both propofol and resveratrol 10 min before hepatic portal vein occlusion. At 1, 3 and 6 h after the reperfusion, 6 rats from each group were sacrificed for histopathological examination of the liver tissue, detection of hepatocyte apoptosis using TUNEL assay, and measurement of Bcl-2, Bax and caspase-3 protein expressions using immunohistochemistry.<br />Results: Compared with normal saline and Tween80, propofol and resveratrol at different doses used alone or in combination all significantly alleviated the hepatic pathologies, lowered the apoptosis index (P<0.05), increased Bcl-2 expression (P<0.05), and reduced Bax and caspase-3 expressions in the liver tissues following HIRI (P<0.05). Compared with low doses of propofol and resveratrol used alone, their combination showed more obvious protective effects against hepatocyte apoptosis (P<0.05), but at higher doses, propofol and resveratrol either alone or in combination produced similar effects.<br />Conclusions: Propofol and resveratrol can suppress HIRI-induced hepatocyte apoptosis by up-regulating Bcl-2 and down-regulating Bax and caspase-3 expressions, and their combined use can reduce the effective doses of the drugs.
- Subjects :
- Animals
Caspase 3 metabolism
Hepatocytes drug effects
Ischemic Preconditioning methods
Male
Proto-Oncogene Proteins c-bcl-2 metabolism
Rats
Rats, Sprague-Dawley
Resveratrol
bcl-2-Associated X Protein metabolism
Apoptosis drug effects
Liver Diseases pathology
Propofol pharmacology
Reperfusion Injury pathology
Stilbenes pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 33
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 23353162