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Characterization of heat shock protein 70 gene from Haemonchus contortus and its expression and promoter analysis in Caenorhabditis elegans.
- Source :
-
Parasitology [Parasitology] 2013 May; Vol. 140 (6), pp. 683-94. Date of Electronic Publication: 2013 Jan 29. - Publication Year :
- 2013
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Abstract
- Haemonchus contortus infections in small ruminants are of major economic importance worldwide. Heat shock proteins (HSPs) are a family of molecular chaperones that play important roles in the process of invasion and survival of nematodes. Although HSP70 has been identified in several parasitic nematodes, little is known of its distribution and function in Haemonchus contortus. The aims of this study were to characterize HSP70 from Haemonchus contortus (designed as Hc-hsp70), express Hc-hsp70 and analyse the promoter activity in Caenorhabditis elegans. Bioinformatic analysis revealed that the open reading frame of the Hc-hsp70 cDNA encodes a 646-amino acid peptide, which is highly conserved in comparison to HSP70 in other nematodes. Phylogenetic analysis indicated that H. contortus is closely related to Caenorhabditis. The 5'-flanking region promoted green fluorescence protein (GFP) expression in the intestine in all larval stages and adult with 2 expression patterns in C. elegans. Expression of Hc-hsp70 mRNA transcripts in C. elegans increased following 2, 4, 6 h of heat shock and peaked at 4 h. However, its expression induced down-regulation of hsp-1 of C. elegans. These results suggest that the H. contortus hsp70 might have a similar function to that of C. elegans hsp-1.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Caenorhabditis elegans cytology
Caenorhabditis elegans metabolism
Chromosome Mapping
Computational Biology
DNA, Helminth chemistry
DNA, Helminth genetics
Down-Regulation
Escherichia coli genetics
Escherichia coli metabolism
Feces parasitology
Female
HSP70 Heat-Shock Proteins metabolism
Haemonchus metabolism
Helminth Proteins genetics
Helminth Proteins metabolism
Hot Temperature
Larva
Male
Molecular Sequence Data
Phylogeny
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sheep
Caenorhabditis elegans genetics
Gene Expression Regulation
HSP70 Heat-Shock Proteins genetics
Haemonchus genetics
Promoter Regions, Genetic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8161
- Volume :
- 140
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 23360558
- Full Text :
- https://doi.org/10.1017/S0031182012002168