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The mechanism of effective electroacupuncture on T cell response in rats with experimental autoimmune encephalomyelitis.
- Source :
-
PloS one [PLoS One] 2013; Vol. 8 (1), pp. e51573. Date of Electronic Publication: 2013 Jan 28. - Publication Year :
- 2013
-
Abstract
- Previously, we demonstrated that electroacupuncture (EA) decreased lymphocyte infiltration into the spinal cords of rats presenting with experimental autoimmune encephalomyelitis (EAE), a disease model used in the study of multiple sclerosis (MS). The aim of this study was to characterize the effects of EA on the EAE. Female Lewis rats were divided into either CFA, EAE, EA, or injection with naloxone after electroacupuncture (NAL) groups. Electroacupuncture was administered every day for 21 days. To evaluate proliferation and apoptosis, lymphocytes from rats presenting with EAE were collected and cultured with β-endorphin. Immunohistochemisty, flow cytometry and radio-immunity methods were applied to detect the expression of β-endorphin. Results presented in this report demonstrate that the beneficial anti-inflammatory effects of EA on EAE were related to β-endorphin production that balances the Thl/Th2 and Th17/Treg responses. These results suggest that β-endorphin could be an important component in the development of EA-based therapies used for the treatment of EAE.
- Subjects :
- Animals
Apoptosis drug effects
Apoptosis immunology
Cell Proliferation drug effects
Encephalomyelitis, Autoimmune, Experimental pathology
Female
Humans
Multiple Sclerosis pathology
Multiple Sclerosis therapy
Naloxone pharmacology
Rats
Rats, Inbred Lew
T-Lymphocytes, Regulatory drug effects
T-Lymphocytes, Regulatory immunology
Th1-Th2 Balance drug effects
Th17 Cells cytology
Th17 Cells drug effects
Th17 Cells immunology
beta-Endorphin pharmacology
Electroacupuncture
Encephalomyelitis, Autoimmune, Experimental immunology
Encephalomyelitis, Autoimmune, Experimental therapy
Multiple Sclerosis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23382807
- Full Text :
- https://doi.org/10.1371/journal.pone.0051573