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[Effect of mulberry leaves extracts on glucose uptake of insulin-resistant HepG2 cells and the mechanism].
- Source :
-
Yao xue xue bao = Acta pharmaceutica Sinica [Yao Xue Xue Bao] 2012 Nov; Vol. 47 (11), pp. 1452-6. - Publication Year :
- 2012
-
Abstract
- The effect and mechanism of mulberry leaves extracts (MLE) on glucose uptake of insulin-resistant HepG2 cells in vitro was explored. The insulin resistant models of HepG2 were induced by high concentration of insulin for 24 h. The models were incubated in a buffer containing mulberry leaves extracts. The glucose consumption was detected by glucose assay kits and the AMP-activated protein kinase (AMPK), Akt activation was examined by Western blotting. Mulberry leaves polysaccharides, mulberry leaves flavonoids and mulberry leaves extracts advanced glucose uptake of insulin-resistant HepG2 cells; Mulberry leaves extracts enhance phosphorylation of AMPK. Mulberry leaves extracts do not change the phosphorylation status of Akt. The glucose consumptions of insulin resistant model of HepG2 were promoted by mulberry leaves extracts. MLE stimulates HepG2 cell AMPK activity acutely without changing the Akt activity.
- Subjects :
- Cell Proliferation drug effects
Drugs, Chinese Herbal isolation & purification
Flavonoids isolation & purification
Flavonoids pharmacology
Hep G2 Cells
Humans
Hypoglycemic Agents isolation & purification
Insulin Resistance
Phosphorylation
Plant Extracts pharmacology
Plant Leaves chemistry
Plants, Medicinal chemistry
Polysaccharides isolation & purification
Polysaccharides pharmacology
Proto-Oncogene Proteins c-akt metabolism
AMP-Activated Protein Kinases metabolism
Drugs, Chinese Herbal pharmacology
Glucose metabolism
Hypoglycemic Agents pharmacology
Morus chemistry
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0513-4870
- Volume :
- 47
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Yao xue xue bao = Acta pharmaceutica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 23387076