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Interference of silibinin with IGF-1R signalling pathways protects human epidermoid carcinoma A431 cells from UVB-induced apoptosis.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2013 Mar 08; Vol. 432 (2), pp. 314-9. Date of Electronic Publication: 2013 Feb 08. - Publication Year :
- 2013
-
Abstract
- Ultraviolet B (UVB) from sunlight is a major cause of cutaneous lesion. Silibinin, a traditional hepatic protectant, elicits protective effects against UVB-induced cellular damage. In A431 cells, the insulin-like growth factor-1 receptor (IGF-1R) was markedly up-regulated by UVB irradiation. The activation of the IGF-1R signalling pathways contributed to apoptosis of the cells rather than rescuing the cells from death. Up-regulated IGF-1R stimulated downstream mitogen-activated protein kinases (MAPKs), such as c-Jun N-terminal kinases (JNK) and extracellular signal-regulated protein kinases 1/2 (ERK1/2). The subsequent activation of caspase-8 and caspase-3 led to apoptosis. The activation of IGF-1R signalling pathways is the cause of A431 cell death. The pharmacological inhibitors and the small interfering RNA (siRNA) targeting IGF-1R suppressed the downstream activation of JNK/ERK-caspases to help the survival of the UVB-irradiated A431 cells. Indeed, silibinin treatment suppressed the IGF-1R-JNK/ERK pathways and thus protected the cells from UVB-induced apoptosis.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Subjects :
- Apoptosis radiation effects
Caspase 8 metabolism
Caspase 9 metabolism
Cell Line, Tumor
Down-Regulation
Enzyme Activation
Humans
MAP Kinase Signaling System drug effects
RNA Interference
Receptor, IGF Type 1 metabolism
Signal Transduction
Silybin
Apoptosis drug effects
Receptor, IGF Type 1 antagonists & inhibitors
Silymarin pharmacology
Skin drug effects
Skin radiation effects
Ultraviolet Rays adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 432
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 23396055
- Full Text :
- https://doi.org/10.1016/j.bbrc.2013.01.109