Utility of OCT3/4, TSPY and β-catenin as biological markers for gonadoblastoma formation and malignant germ cell tumor development in dysgenetic gonads.

Authors :: Palma I
Garibay N
Pena-Yolanda R
Contreras A
Raya A
Dominguez C
Romero M
Aristi G
Queipo G
Source :: Disease markers [Dis Markers] 2013; Vol. 34 (6), pp. 419-24.
Publication Year :: 2013
Abstract: Background: Gonadoblastoma (GB) is regarded as an in situ form of germ cell tumor in dysgenetic gonads, and 30% of patients with GB develop a dysgerminoma/seminoma tumor.<br />Objective: Determine whether OCT3/4 and β-catenin are expressed in dysgenetic gonads before GB development and whether TSPY participates in the OCT3/4-β-catenin pathways in the malignant invasive behavior.<br />Methods: dysgenetic gonads of Disorders of sex differentiation (DSD) patients with mixed gonadal dysgenesis were analyzed by immunohistochemistry and immunofluorescence for comparison with GB and dysgerminoma/seminoma.<br />Results: Our results suggest that the development of GB is secondary to the interaction of OCT3/4 and TSPY, that β-catenin does not participate in this process.<br />Conclusions: The use of this biological markers detects the potential high risk gonads.

Subjects :: Case-Control Studies
Child
Dysgerminoma diagnosis
Humans
Biomarkers, Tumor analysis
Cell Cycle Proteins analysis
Gonadal Dysgenesis diagnosis
Gonadoblastoma diagnosis
Neoplasms, Gonadal Tissue diagnosis
Octamer Transcription Factor-3 analysis
beta Catenin analysis

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