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Copper regulates primary root elongation through PIN1-mediated auxin redistribution.
- Source :
-
Plant & cell physiology [Plant Cell Physiol] 2013 May; Vol. 54 (5), pp. 766-78. Date of Electronic Publication: 2013 Feb 08. - Publication Year :
- 2013
-
Abstract
- The heavy metal copper (Cu) is an essential microelement required for normal plant growth and development, but it inhibits primary root growth when in excess. The mechanism underlying how excess Cu functions in this process remains to be further elucidated. Here, we report that a higher concentration of CuSO4 inhibited primary root elongation of Arabidopsis seedlings by affecting both the elongation and meristem zones. In the meristem zone, meristematic cell division potential was reduced by excess Cu. Further experiments showed that Cu can modulate auxin distribution, resulting in higher auxin activities in both the elongation and meristem zones of Cu-treated roots based on DR5::GUS expression patterns. This Cu-mediated auxin redistribution was shown to be responsible for Cu-mediated inhibition of primary root elongation. Additional genetic and physiological data demonstrated that it was PINFORMED1 (PIN1), but not PIN2 or AUXIN1 (AUX1), that regulated this process. However, Cu-induced hydrogen peroxide accumulation did not contribute to Cu-induced auxin redistribution for inhibition of root elongation. When the possible role of ethylene in this process was analyzed, Cu had a similar impact on the root elongation of both the wild type and the ein2-1 mutant, implying that Cu-mediated inhibition of primary root elongation was not due to the ethylene signaling pathway.
- Subjects :
- Arabidopsis drug effects
Biological Transport drug effects
Copper Sulfate pharmacology
Ethylenes metabolism
Hydrogen Peroxide metabolism
Meristem drug effects
Meristem growth & development
Plant Roots drug effects
Potassium Iodide pharmacology
Seedlings drug effects
Seedlings metabolism
Signal Transduction drug effects
Arabidopsis growth & development
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Copper pharmacology
Indoleacetic Acids metabolism
Membrane Transport Proteins metabolism
Plant Roots growth & development
Plant Roots metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1471-9053
- Volume :
- 54
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Plant & cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 23396597
- Full Text :
- https://doi.org/10.1093/pcp/pct030