Back to Search
Start Over
Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentine bond properties.
- Source :
-
Journal of dentistry [J Dent] 2013 May; Vol. 41 (5), pp. 464-74. Date of Electronic Publication: 2013 Feb 08. - Publication Year :
- 2013
-
Abstract
- Objectives: The objective of this study was to investigate the effects of dentine primer containing dual antibacterial agents, namely, 12-methacryloyloxydodecylpyridinium bromide (MDPB) and nanoparticles of silver (NAg), on dentine bond strength, dental plaque microcosm biofilm response, and fibroblast cytotoxicity for the first time.<br />Methods: Scotchbond Multi-Purpose (SBMP) was used as the parent bonding agent. Four primers were tested: SBMP primer control (referred to as "P"), P+5% MDPB, P+0.05% NAg, and P+5% MDPB+0.05% NAg. Dentine shear bond strengths were measured using extracted human teeth. Biofilms from the mixed saliva of 10 donors were cultured to investigate metabolic activity, colony-forming units (CFU), and lactic acid production. Human fibroblast cytotoxicity of the four primers was tested in vitro.<br />Results: Incorporating MDPB and NAg into primer did not reduce dentine bond strength compared to control (p>0.1). SEM revealed well-bonded adhesive-dentine interfaces with numerous resin tags. MDPB or NAg each greatly reduced biofilm viability and acid production, compared to control. Dual agents MDPB+NAg had a much stronger effect than either agent alone (p<0.05), increasing inhibition zone size and reducing metabolic activity, CFU and lactic acid by an order of magnitude, compared to control. There was no difference in cytotoxicity between commercial control and antibacterial primers (p>0.1).<br />Conclusions: The method of using dual agents MDPB+NAg in the primer yielded potent antibacterial properties. Hence, this method may be promising to combat residual bacteria in tooth cavity and invading bacteria at the margins. The dual agents MDPB+NAg may have wide applicability to other adhesives, composites, sealants and cements to inhibit biofilms and caries.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Anti-Bacterial Agents pharmacology
Anti-Bacterial Agents toxicity
Bacterial Load drug effects
Cells, Cultured
Dental Plaque microbiology
Gingiva cytology
Gingiva drug effects
Humans
Lactic Acid metabolism
Materials Testing
Microbial Viability drug effects
Microscopy, Electron, Scanning
Pyridinium Compounds pharmacology
Pyridinium Compounds toxicity
Resin Cements chemistry
Saliva microbiology
Shear Strength
Silver pharmacology
Silver toxicity
Streptococcus drug effects
Streptococcus mutans drug effects
Stress, Mechanical
Surface Properties
Anti-Bacterial Agents chemistry
Biofilms drug effects
Dental Bonding
Dentin ultrastructure
Fibroblasts drug effects
Nanoparticles chemistry
Pyridinium Compounds chemistry
Silver chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-176X
- Volume :
- 41
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of dentistry
- Publication Type :
- Academic Journal
- Accession number :
- 23402889
- Full Text :
- https://doi.org/10.1016/j.jdent.2013.02.001