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Effects of salinomycin on human bone marrow-derived mesenchymal stem cells in vitro.
- Source :
-
Toxicology letters [Toxicol Lett] 2013 Apr 26; Vol. 218 (3), pp. 207-14. Date of Electronic Publication: 2013 Feb 12. - Publication Year :
- 2013
-
Abstract
- Various hypotheses on the origin of cancer stem cells (CSCs) exist, including that CSCs develop from transformed human bone marrow mesenchymal stem cells (hBMSC). Since the polyether antibiotic salinomycin selectively kills CSCs, the present study aims to elucidate the effects of salinomycin on normal hBMSC. The immunophenotype of hBMSC after salinomycin exposure was observed by flow cytometry. The multi-differentiation capacity of hBMSC was evaluated by Oil Red O and van Kossa staining. Cytotoxic effects of salinomycin were monitored by the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay. Furthermore, spheroid formation and migration capacity were assessed. There were no differences in the immunophenotype and multi-differentiation capacity of hBMSC induced by salinomycin treatment. Cytotoxic effects were observed at concentrations of 30 μM and above. Neither the migration capability nor the ability to form spheroids was affected. Essential functional properties of hBMSC were unaffected by salinomycin. However, dose-dependent cytotoxicity effects could be observed. Overall, low dose salinomycin showed no negative effects on hBMSC. Since mesenchymal stem cells from various sources respond differently, further in vitro studies are needed to clarify the effect of salinomycin on tissue-specific stem cells.<br /> (Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Bone Marrow Cells immunology
Bone Marrow Cells pathology
Cell Differentiation drug effects
Cell Line
Cell Movement drug effects
Cell Shape drug effects
Cell Survival drug effects
Dose-Response Relationship, Drug
Flow Cytometry
Humans
Immunophenotyping methods
Mesenchymal Stem Cells immunology
Mesenchymal Stem Cells pathology
Bone Marrow Cells drug effects
Mesenchymal Stem Cells drug effects
Pyrans toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3169
- Volume :
- 218
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 23410960
- Full Text :
- https://doi.org/10.1016/j.toxlet.2013.02.001