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Accumulation of xenotransplanted canine bone marrow cells in NOD/SCID/γc(null) mice with acute hepatitis induced by CCl4.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 2013 Jul 31; Vol. 75 (7), pp. 847-55. Date of Electronic Publication: 2013 Feb 15. - Publication Year :
- 2013
-
Abstract
- Bone marrow cell infusion (BMI) has recently been suggested as an effective therapy for refractory liver disease; however, the efficiency of BMI using canine bone marrow cells (cBMCs) has not been reported. We evaluated the accumulation potential of cBMCs in a mouse model of acute liver failure. Acute hepatitis was induced by carbon tetrachloride (CCl4) treatment in NOD/SCID/γc(null)(NOG) mice and wild-type (WT) C57BL mice, and the characteristics of liver dysfunction and the degree of hepatic injury and regeneration were compared between the two mouse models. Next, female CCl4-treated NOG mice were xenotransplanted with male PKH26-labeled cBMCs, and the potential of cBMCs to accumulate in injured liver tissue compartments was examined. Fluorescence microscopy was performed to histologically detect the infused cBMCs, and DNA polymerase chain reaction was performed for detection of the male Y chromosome (SRY gene) in the recipient female NOG mice. The number of PKH26-positive cBMCs transplanted in the liver tissue gradually increased in the NOG mice. The infused cBMCs were located in the necrotic area of the liver at an early stage after transplantation, and most had accumulated a week after transplantation. However, the therapeutic efficacy of the xenotransplantation remained unclear, because no significant differences were observed concerning the extent liver injury and regeneration between the cBMC-transplanted and saline control mice. These results suggest that cBMCs will specifically accumulate in injured liver tissue and that BMC transplantation may have the potential to repair liver deficiency.
- Subjects :
- Animals
Dogs
Female
Genes, sry genetics
Male
Mice
Mice, Inbred C57BL
Mice, Inbred NOD
Mice, SCID
Microscopy, Fluorescence
Bone Marrow Transplantation methods
Carbon Tetrachloride toxicity
Chemical and Drug Induced Liver Injury drug therapy
Liver cytology
Liver Regeneration physiology
Transplantation, Heterologous methods
Subjects
Details
- Language :
- English
- ISSN :
- 1347-7439
- Volume :
- 75
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 23411484
- Full Text :
- https://doi.org/10.1292/jvms.12-0530