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Fetal hepatic progenitors support long-term expansion of hematopoietic stem cells.
- Source :
-
Experimental hematology [Exp Hematol] 2013 May; Vol. 41 (5), pp. 479-490.e4. Date of Electronic Publication: 2013 Feb 13. - Publication Year :
- 2013
-
Abstract
- We have developed a coculture system that establishes DLK(+) fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK(+) fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK(+) cells, but not their conditioned medium, continuously and significantly (>20-fold) expanded both hematopoietic stem and progenitor cells. Physical contact between HSCs and DLK(+) cells was crucial to maintaining this long-term expansion. Similar HSC expansion (approximately sevenfold) was achieved in cocultures using a serum-free, low cytokine- containing medium. In contrast, DLK(-) cells are incapable of expanding hematopoietic cells, demonstrating that hepatic progenitors are the principle supportive cells for HSC expansion in the fetal liver.<br /> (Copyright © 2013 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Calcium-Binding Proteins
Cell Adhesion drug effects
Cells, Cultured
Coculture Techniques
Culture Media, Conditioned pharmacology
Culture Media, Serum-Free pharmacology
Cytokines pharmacology
Fetus
Flow Cytometry
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Hematopoietic Stem Cells metabolism
Immunohistochemistry
Intercellular Signaling Peptides and Proteins metabolism
Liver cytology
Liver embryology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Stem Cells drug effects
Stem Cells metabolism
Time Factors
Cell Culture Techniques methods
Cell Proliferation
Hematopoietic Stem Cells cytology
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2399
- Volume :
- 41
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Experimental hematology
- Publication Type :
- Academic Journal
- Accession number :
- 23415675
- Full Text :
- https://doi.org/10.1016/j.exphem.2013.02.003