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Multiple functions of Kip-related protein5 connect endoreduplication and cell elongation.
- Source :
-
Plant physiology [Plant Physiol] 2013 Apr; Vol. 161 (4), pp. 1694-705. Date of Electronic Publication: 2013 Feb 20. - Publication Year :
- 2013
-
Abstract
- Despite considerable progress in our knowledge regarding the cell cycle inhibitor of the Kip-related protein (KRP) family in plants, less is known about the coordination of endoreduplication and cell differentiation. In animals, the role of cyclin-dependent kinase (CDK) inhibitors as multifunctional factors coordinating cell cycle regulation and cell differentiation is well documented and involves not only the inhibition of CDK/cyclin complexes but also other mechanisms, among them the regulation of transcription. Interestingly, several plant KRPs have a punctuated distribution in the nucleus, suggesting that they are associated with heterochromatin. Here, one of these chromatin-bound KRPs, KRP5, has been studied in Arabidopsis (Arabidopsis thaliana). KRP5 is expressed in endoreduplicating cells, and loss of KRP5 function decreases endoreduplication, indicating that KRP5 is a positive regulator of endoreduplication. This regulation relies on several mechanisms: in addition to its role in cyclin/CDK kinase inhibition previously described, chromatin immunoprecipitation sequencing data combined with transcript quantification provide evidence that KRP5 regulates the transcription of genes involved in cell wall organization. Furthermore, KRP5 overexpression increases chromocenter decondensation and endoreduplication in the Arabidopsis trithorax-related protein5 (atxr5) atxr6 double mutant, which is deficient for the deposition of heterochromatin marks. Hence, KRP5 could bind chromatin to coordinately control endoreduplication and chromatin structure and allow the expression of genes required for cell elongation.
- Subjects :
- Arabidopsis genetics
Arabidopsis Proteins genetics
Cyclin-Dependent Kinase Inhibitor Proteins genetics
Cyclins metabolism
Genes, Plant genetics
Heterochromatin metabolism
Models, Biological
Mutation genetics
Protein Binding genetics
Protein Transport
Seedlings metabolism
Transcriptional Activation genetics
Arabidopsis cytology
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Cyclin-Dependent Kinase Inhibitor Proteins metabolism
Endoreduplication
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 161
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 23426196
- Full Text :
- https://doi.org/10.1104/pp.112.212357