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The effect of repeated administration of methotrexate (MTX) on rat ovary: measurement of serum antimullerian hormone (AMH) levels.
- Source :
-
Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology [Gynecol Endocrinol] 2013 Mar; Vol. 29 (3), pp. 226-9. - Publication Year :
- 2013
-
Abstract
- Objective: To evaluate the possible effect of methotrexate (MTX) on rat ovaries by measuring serum antimullerian hormone (AMH), the novel marker of the ovarian reserve.<br />Methods: Pretreatment serum AMH levels were measured in 15 Wistar albino rats. MTX was given in 1 mg/kg dose in days 1, 3, 5, and 7. Serum AMH levels were measured twenty-four hours after each MTX administration. Pre- and post-treatment serum AMH levels were compared.<br />Results: Pretreatment median serum AMH was 102.4 ng/mL (25%: 41.9; 75%: 179.8). The median serum AMH levels were 70.6 ng/mL (25%: 54.08; 75%: 125.5); 136.1 ng/mL (25%: 57.3; 75%: 223.09); 121.2 ng/mL (25%: 52.5; 75%: 151.5); and 104.7 ng/mL (25%: 65.8; 75%: 265.5) after the first, second, third, and fourth methotrexate administrations, respectively. The ratio of the final (eighth day) median serum AMH level to the pretreatment median AMH level was 1.27 (25%: 0.84 and 75%: 2.57). Wilcoxon related samples test showed that final AMH was significantly higher as compared to the second day AMH measurement (p = 0.041).<br />Conclusion: MTX administration did not cause a statistically significant change between pretreatment and final serum AMH levels in rats. There was no decrease in AMH levels indicating a decrease in ovarian reserve.
- Subjects :
- Animals
Antimetabolites, Antineoplastic adverse effects
Antirheumatic Agents administration & dosage
Antirheumatic Agents adverse effects
Biomarkers blood
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Female
Immunosuppressive Agents administration & dosage
Immunosuppressive Agents adverse effects
Methotrexate adverse effects
Nucleic Acid Synthesis Inhibitors adverse effects
Primary Ovarian Insufficiency blood
Rats
Rats, Wistar
Reproducibility of Results
Anti-Mullerian Hormone blood
Antimetabolites, Antineoplastic administration & dosage
Methotrexate administration & dosage
Nucleic Acid Synthesis Inhibitors administration & dosage
Ovary drug effects
Primary Ovarian Insufficiency chemically induced
Subjects
Details
- Language :
- English
- ISSN :
- 1473-0766
- Volume :
- 29
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 23428228
- Full Text :
- https://doi.org/10.3109/09513590.2012.738725