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IFNγ/TNFα specific-cells and effector memory phenotype associate with active tuberculosis.
- Source :
-
The Journal of infection [J Infect] 2013 Jun; Vol. 66 (6), pp. 475-86. Date of Electronic Publication: 2013 Feb 24. - Publication Year :
- 2013
-
Abstract
- Objectives: Controversial results were reported on the role of polyfunctional T-cells in tuberculosis (TB). Our aim was to simultaneously characterize the Mycobacterium tuberculosis (Mtb)-specific immune response as cytokine production and memory phenotype by flow cytometry after in vitro stimulation with region of difference 1 ("RD1") recombinant proteins (ESAT-6 and CFP-10) in patients at different TB stage in a low TB endemic country. To assess the specificity of these findings, we evaluated the response to cytomegalovirus (CMV), an unrelated antigen.<br />Methods: We enrolled subjects with active TB, cured TB, latent TB infection (LTBI). Cytokine and phenotype profiles of T-cells from whole blood stimulated with "RD1" proteins and CMV were characterized by multi-parametric flow cytometry.<br />Results: Bifunctional IFNγ(+) TNFα(+) CD4(+) T-cells and effector memory phenotype significantly associated with active TB compared to the LTBI group (p = 0.008, at least p ≤ 0.009 respectively) whereas "RD1"-T-cell response in cured TB and LTBI was characterized by a central memory phenotype (at least p ≤ 0.013 and p ≤ 0.004 respectively vs active TB). In contrast, response to CMV antigen was not associated with a TB-specific status.<br />Conclusion: We identified qualitative associations between Mtb-specific T-cell and TB status in terms of functional capacity and memory status. These immune correlates may be helpful to trace natural history of TB.<br /> (Copyright © 2013 The British Infection Association. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Adult
Antigens, Bacterial immunology
Antigens, Viral immunology
Bacterial Proteins immunology
CD4-Positive T-Lymphocytes metabolism
Cytomegalovirus immunology
Female
Flow Cytometry
Humans
Immunologic Memory
Interferon-gamma immunology
Male
Middle Aged
Phenotype
Tumor Necrosis Factor-alpha immunology
CD4-Positive T-Lymphocytes immunology
Interferon-gamma metabolism
Tuberculosis immunology
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2742
- Volume :
- 66
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of infection
- Publication Type :
- Academic Journal
- Accession number :
- 23462597
- Full Text :
- https://doi.org/10.1016/j.jinf.2013.02.004