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Liquid chromatography-tandem mass spectrometry method for simultaneous quantification of urapidil and aripiprazole in human plasma and its application to human pharmacokinetic study.
- Source :
-
Biomedical chromatography : BMC [Biomed Chromatogr] 2013 Jul; Vol. 27 (7), pp. 916-23. Date of Electronic Publication: 2013 Mar 06. - Publication Year :
- 2013
-
Abstract
- A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of urapidil and aripiprazole in human plasma. A simple liquid-liquid extraction with ethyl acetate was used for the sample preparation. Chromatographic separation was achieved on a Phenomenex C18 (4.6 × 50 mm, 5 µm) column with 0.1% formic acid-acetonitrile (10:90, v/v) as the mobile phase with flow rate of 0.6 mL/min. The quantitation of the target compounds was determined in a positive ion multiple reaction monitoring mode. Calibration plots were linear over the range of 2.0-2503.95 ng/mL for urapidil and 1.0-500.19 ng/mL for aripiprazole. The lower limit of quantitation for urapidil and aripiprazole was 2.0 and 1.0 ng/mL, respectively. Mean recovery was in the range of 69.94-75.62% for both analytes and internal standards. Intra-day and inter-day precisions of the assay at three concentrations were 2.56-5.89% with accuracy of 92.31-97.83% for urapidil, and 3.14-6.84% with accuracy of 91.38-94.42% for aripiprazole. The method was successfully applied to human pharmacokinetic study of urapidil and aripiprazole in healthy human male volunteers.<br /> (Copyright © 2013 John Wiley & Sons, Ltd.)
- Subjects :
- Acetates
Aripiprazole
Drug Stability
Humans
Least-Squares Analysis
Liquid-Liquid Extraction
Male
Piperazines chemistry
Piperazines pharmacokinetics
Quinolones chemistry
Quinolones pharmacokinetics
Reproducibility of Results
Sensitivity and Specificity
Chromatography, Liquid methods
Piperazines blood
Quinolones blood
Tandem Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0801
- Volume :
- 27
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biomedical chromatography : BMC
- Publication Type :
- Academic Journal
- Accession number :
- 23463771
- Full Text :
- https://doi.org/10.1002/bmc.2882