Back to Search
Start Over
Paraquat Resistant1, a Golgi-localized putative transporter protein, is involved in intracellular transport of paraquat.
- Source :
-
Plant physiology [Plant Physiol] 2013 May; Vol. 162 (1), pp. 470-83. Date of Electronic Publication: 2013 Mar 07. - Publication Year :
- 2013
-
Abstract
- Paraquat is one of the most widely used herbicides worldwide. In green plants, paraquat targets the chloroplast by transferring electrons from photosystem I to molecular oxygen to generate toxic reactive oxygen species, which efficiently induce membrane damage and cell death. A number of paraquat-resistant biotypes of weeds and Arabidopsis (Arabidopsis thaliana) mutants have been identified. The herbicide resistance in Arabidopsis is partly attributed to a reduced uptake of paraquat through plasma membrane-localized transporters. However, the biochemical mechanism of paraquat resistance remains poorly understood. Here, we report the identification and characterization of an Arabidopsis paraquat resistant1 (par1) mutant that shows strong resistance to the herbicide without detectable developmental abnormalities. PAR1 encodes a putative l-type amino acid transporter protein localized to the Golgi apparatus. Compared with the wild-type plants, the par1 mutant plants show similar efficiency of paraquat uptake, suggesting that PAR1 is not directly responsible for the intercellular uptake of paraquat. However, the par1 mutation caused a reduction in the accumulation of paraquat in the chloroplast, suggesting that PAR1 is involved in the intracellular transport of paraquat into the chloroplast. We identified a PAR1-like gene, OsPAR1, in rice (Oryza sativa). Whereas the overexpression of OsPAR1 resulted in hypersensitivity to paraquat, the knockdown of its expression using RNA interference conferred paraquat resistance on the transgenic rice plants. These findings reveal a unique mechanism by which paraquat is actively transported into the chloroplast and also provide a practical approach for genetic manipulations of paraquat resistance in crops.
- Subjects :
- Arabidopsis cytology
Arabidopsis drug effects
Arabidopsis genetics
Arabidopsis Proteins genetics
Basic Helix-Loop-Helix Transcription Factors genetics
Biological Transport
Chlorophyll metabolism
Chromosome Mapping
Gene Expression
Gene Expression Regulation, Plant drug effects
Herbicide Resistance
Mutagenesis, Insertional
Oryza genetics
Oryza metabolism
Phenotype
Plant Leaves cytology
Plant Leaves drug effects
Plant Leaves genetics
Plant Leaves metabolism
Plants, Genetically Modified
Seedlings cytology
Seedlings drug effects
Seedlings genetics
Seedlings metabolism
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Basic Helix-Loop-Helix Transcription Factors metabolism
Chloroplasts metabolism
Golgi Apparatus metabolism
Herbicides metabolism
Paraquat metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 162
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 23471133
- Full Text :
- https://doi.org/10.1104/pp.113.213892