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Rho participates in chemoreceptor-induced changes in morphology to hair bundle mechanoreceptors of the sea anemone, Nematostella vectensis.
- Source :
-
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2013 Jun; Vol. 165 (2), pp. 139-48. Date of Electronic Publication: 2013 Mar 06. - Publication Year :
- 2013
-
Abstract
- Adjustable hair bundle mechanoreceptors located on anemone tentacles detect movements of nearby, swimming prey. The hair bundles are formed by numerous actin-based stereocilia that converge onto a single, central kinocilium. Interestingly, morphological and functional changes to the hair bundles are induced by activating chemoreceptors that bind prey-derived N-acetylated sugars and proline, respectively. Morphological changes to the hair bundles involve alterations to the actin cytoskeleton of stereocilia. A pharmacological activation of Rho induces hair bundles to elongate to lengths comparable to those normally induced by exposure to N-acetylneuraminic acid (NANA) and prevents shortening of hair bundles normally induced by proline. Rho inhibition prevents NANA-induced elongation, but does not prevent proline-induced shortening of hair bundles. Western blots feature a band similar in mass to that predicted for a Rho homolog in the genome of Nematostella. Immunocytochemistry localizes Rho in stereocilia of the hair bundle. Anemone hair bundles arise from multicellular complexes. Data from experiments using heptanol, a gap junction uncoupler, indicate that cell-cell communication is required in order for activated chemoreceptors to induce morphological changes to the hair bundles.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Subjects :
- Actin Cytoskeleton metabolism
Amino Acid Sequence
Animals
Blotting, Western
Calcimycin pharmacology
Calcium Ionophores pharmacology
Cell Communication drug effects
Cell Communication physiology
Chemoreceptor Cells drug effects
Chemoreceptor Cells metabolism
Epidermal Cells
Epidermis metabolism
Gap Junctions drug effects
Gap Junctions metabolism
Gap Junctions physiology
Heptanol pharmacology
Immunohistochemistry
Mechanoreceptors cytology
Mechanoreceptors metabolism
Models, Biological
Molecular Sequence Data
N-Acetylneuraminic Acid pharmacology
Sea Anemones cytology
Sea Anemones metabolism
Signal Transduction drug effects
rho GTP-Binding Proteins genetics
Chemoreceptor Cells physiology
Mechanoreceptors physiology
Sea Anemones physiology
rho GTP-Binding Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1531-4332
- Volume :
- 165
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
- Publication Type :
- Academic Journal
- Accession number :
- 23474255
- Full Text :
- https://doi.org/10.1016/j.cbpa.2013.03.003