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QuaNCAT: quantitating proteome dynamics in primary cells.
- Source :
-
Nature methods [Nat Methods] 2013 Apr; Vol. 10 (4), pp. 343-6. Date of Electronic Publication: 2013 Mar 10. - Publication Year :
- 2013
-
Abstract
- Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
- Subjects :
- Amino Acids
CD4-Positive T-Lymphocytes drug effects
Calcium Ionophores pharmacology
Carcinogens pharmacology
Chromatography, Liquid methods
Humans
Ionomycin pharmacology
Isotope Labeling
Phorbol Esters pharmacology
Sensitivity and Specificity
Tandem Mass Spectrometry methods
CD4-Positive T-Lymphocytes metabolism
Gene Expression Regulation physiology
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1548-7105
- Volume :
- 10
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nature methods
- Publication Type :
- Academic Journal
- Accession number :
- 23474466
- Full Text :
- https://doi.org/10.1038/nmeth.2401