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Immunohistochemical profile of ezrin and radixin in human liver epithelia during fetal development and pediatric cholestatic diseases.
- Source :
-
Clinics and research in hepatology and gastroenterology [Clin Res Hepatol Gastroenterol] 2013 Apr; Vol. 37 (2), pp. 142-51. Date of Electronic Publication: 2013 Mar 16. - Publication Year :
- 2013
-
Abstract
- Aim: Ezrin and radixin are actin-binding proteins that contribute to the integrity of epithelia. Abnormalities of bile secretion occur primarily in cholestatic liver diseases and are associated with changes in cell cytoskeleton. Expression of these proteins during liver development and in cholestatic liver diseases remains poorly investigated.<br />Methods: Ezrin and radixin expression was analyzed in fetal, adult and pediatric cholestatic human liver (i.e. biliary atresia, sclerosing cholangitis) by immunohistochemistry.<br />Results: In adult and fetal livers, ezrin was expressed exclusively in the cells of the biliary lineage (i.e. biliary epithelial cells and ductal cells) whereas radixin was located not only in hepatocytes but also in cells of the biliary lineage. In the lobule of mature livers, radixin displayed a zonal distribution with predominant expression in the periportal region. In cholestatic diseases, both proteins were expressed in cells of the ductular reaction. An aberrant expression of ezrin was detected in hepatocytes of cirrhotic nodules with a CK7-positive pattern and in malignant hepatocytes in a course of cholestatic disease toward cancer.<br />Conclusions: Among the components of the liver epithelial cells, ezrin was exclusively expressed in biliary phenotype cells, while radixin was found in biliary and hepatocytic lineages, with a periportal zonal expression. In cholestatic diseases, ezrin was expressed in hepatocytes supporting the appearance of a biliary phenotype.<br /> (Copyright © 2013. Published by Elsevier Masson SAS.)
- Subjects :
- Aborted Fetus
Adolescent
Bile Duct Diseases metabolism
Bile Duct Diseases pathology
Biliary Tract cytology
Cell Transformation, Neoplastic metabolism
Cell Transformation, Neoplastic pathology
Child
Child, Preschool
Cytoskeletal Proteins genetics
Epithelium metabolism
Humans
Immunohistochemistry
Infant
Keratin-7 metabolism
Liver embryology
Membrane Proteins genetics
RNA, Messenger metabolism
Retrospective Studies
Cytoskeletal Proteins metabolism
Epithelial Cells metabolism
Hepatocytes metabolism
Liver cytology
Membrane Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2210-741X
- Volume :
- 37
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Clinics and research in hepatology and gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 23507543
- Full Text :
- https://doi.org/10.1016/j.clinre.2013.02.001