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Tobramycin is a suppressor of premature termination codons.
- Source :
-
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society [J Cyst Fibros] 2013 Dec; Vol. 12 (6), pp. 806-11. Date of Electronic Publication: 2013 Mar 27. - Publication Year :
- 2013
-
Abstract
- Premature translation terminations (PTCs) constitute the molecular basis of many genetic diseases, including cystic fibrosis, as they lead to the synthesis of truncated non-functional or partially functional protein. Suppression of translation terminations at PTCs (read-through) has been developed as a therapeutic strategy to restore full-length protein in several genetic diseases. Phenotypic consequences of PTCs can be exacerbated by the nonsense-mediated mRNA decay (NMD) pathway that detects and degrades mRNA containing PTC. Modulation of NMD, therefore, is also of interest as a potential target for the suppression therapy. Tobramycin is an aminoglycoside antibiotic, normally used to treat Pseudomonas aeruginosa pulmonary infection in CF patients. In the present study, by using yeast as a genetic system, we have examined the ability of Tobramycin to suppress PTCs as a function of the presence or absence of NMD. Results demonstrate that Tobramycin exhibits read-through ability on PTCs and preferentially in absence of NMD.<br /> (Copyright © 2013 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Codon, Nonsense drug effects
Codon, Nonsense metabolism
Cystic Fibrosis Transmembrane Conductance Regulator genetics
Genes, Reporter
Gentamicins pharmacology
Models, Genetic
Nonsense Mediated mRNA Decay genetics
Saccharomyces cerevisiae genetics
Suppression, Genetic genetics
Anti-Bacterial Agents pharmacology
Anti-Bacterial Agents therapeutic use
Codon, Nonsense genetics
Cystic Fibrosis drug therapy
Cystic Fibrosis genetics
Nonsense Mediated mRNA Decay drug effects
Suppression, Genetic drug effects
Tobramycin pharmacology
Tobramycin therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5010
- Volume :
- 12
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
- Publication Type :
- Academic Journal
- Accession number :
- 23540394
- Full Text :
- https://doi.org/10.1016/j.jcf.2013.02.007