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Single cell time-lapse imaging of focus formation by the DNA damage-response protein 53BP1 after UVC irradiation of human pancreatic cancer cells.
- Source :
-
Anticancer research [Anticancer Res] 2013 Apr; Vol. 33 (4), pp. 1373-7. - Publication Year :
- 2013
-
Abstract
- We have previously demonstrated that ultraviolet (UV) light treatment is effective against various types of cancer cells expressing fluorescent proteins. In order to further understand the efficacy of UV treatment of cancer cells, we determined the kinetics of focus formation by imaging of a DNA damage-response (DDR) protein after UVC irradiation of human pancreatic cancer cells. A fusion protein consisting of the DDR protein 53BP1 and green fluorescent protein (GFP) (GFP-53BP1) was used as a live-cell imaging marker for cellular response after UVC irradiation. GFP-53BP1 foci were observed after UVC irradiation of MiaPaCa-2 human pancreatic cancer cells. During live-cell imaging, GFP-53BP1 foci were observed in the cells within 15 min after UVC irradiation, and some of the foci remained stable for at least three hours. GFP-53BP1 focus formation was observed in the pancreatic-cancer cells irradiated by 25-200 J/m(2) UVC. Our results indicate that an early response to DNA damage caused by UVC irradiation can be visualized by increased GFP-53BP1 focus formation by pancreatic cancer cells.
- Subjects :
- Animals
Cell Nucleus metabolism
Cell Nucleus pathology
Cell Nucleus radiation effects
DNA Repair radiation effects
Dose-Response Relationship, Radiation
Humans
Mice
Mice, Nude
Microscopy, Confocal
Pancreatic Neoplasms metabolism
Pancreatic Neoplasms radiotherapy
Tumor Cells, Cultured
Tumor Suppressor p53-Binding Protein 1
DNA Damage radiation effects
Green Fluorescent Proteins metabolism
Intracellular Signaling Peptides and Proteins metabolism
Pancreatic Neoplasms pathology
Time-Lapse Imaging
Ultraviolet Rays adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7530
- Volume :
- 33
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 23564775