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Dietary fat content and fiber type modulate hind gut microbial community and metabolic markers in the pig.
- Source :
-
PloS one [PLoS One] 2013; Vol. 8 (4), pp. e59581. Date of Electronic Publication: 2013 Apr 03. - Publication Year :
- 2013
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Abstract
- Obesity leads to changes in the gut microbial community which contribute to the metabolic dysregulation in obesity. Dietary fat and fiber affect the caloric density of foods. The impact of dietary fat content and fiber type on the microbial community in the hind gut is unknown. Effect of dietary fat level and fiber type on hindgut microbiota and volatile fatty acid (VFA) profiles was investigated. Expression of metabolic marker genes in the gut, adipose tissue and liver was determined. A 2 × 2 experiment was conducted in pigs fed at two dietary fat levels (5% or 17.5% swine grease) and two fiber types (4% inulin, fermentable fructo-oligosaccharide or 4% solka floc, non-fermentable cellulose). High fat diets (HFD) resulted in a higher (P<0.05) total body weight gain, feed efficiency and back fat accumulation than the low fat diet. Feeding of inulin, but not solka floc, attenuated (P<0.05) the HFD-induced higher body weight gain and fat mass accumulation. Inulin feeding tended to lead to higher total VFA production in the cecum and resulted in a higher (P<0.05) expression of acyl coA oxidase (ACO), a marker of peroxisomal β-oxidation. Inulin feeding also resulted in lower expression of sterol regulatory element binding protein 1c (SREBP-1c), a marker of lipid anabolism. Bacteria community structure characterized by DGGE analysis of PCR amplified 16S rRNA gene fragments showed that inulin feeding resulted in greater bacterial population richness than solka floc feeding. Cluster analysis of pairwise Dice similarity comparisons of the DGGE profiles showed grouping by fiber type but not the level of dietary fat. Canonical correspondence analysis (CCA) of PCR- DGGE profiles showed that inulin feeding negatively correlated with back fat thickness. This study suggests a strong interplay between dietary fat level and fiber type in determining susceptibility to obesity.
- Subjects :
- Animals
Back anatomy & histology
Bacteroides genetics
Bifidobacterium genetics
Biomarkers metabolism
Cecum enzymology
Cellulose administration & dosage
Cellulose metabolism
Denaturing Gradient Gel Electrophoresis
Diet, High-Fat
Dietary Fats
Dietary Fiber
Female
Fermentation
Inulin administration & dosage
Inulin metabolism
Molecular Typing
Principal Component Analysis
RNA, Bacterial genetics
RNA, Ribosomal, 16S genetics
Sequence Analysis, RNA
Subcutaneous Fat anatomy & histology
Subcutaneous Fat metabolism
Sus scrofa growth & development
Sus scrofa metabolism
Acyl-CoA Oxidase metabolism
Cecum microbiology
Metagenome
Sterol Regulatory Element Binding Protein 1 metabolism
Sus scrofa microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23573202
- Full Text :
- https://doi.org/10.1371/journal.pone.0059581