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Effect of water-ageing on dentine bond strength and anti-biofilm activity of bonding agent containing new monomer dimethylaminododecyl methacrylate.
- Source :
-
Journal of dentistry [J Dent] 2013 Jun; Vol. 41 (6), pp. 504-13. Date of Electronic Publication: 2013 Apr 10. - Publication Year :
- 2013
-
Abstract
- Objectives: The objectives of this study were to develop bonding agent containing a new antibacterial monomer dimethylaminododecyl methacrylate (DMADDM) as well as nanoparticles of silver (NAg) and nanoparticles of amorphous calcium phosphate (NACP), and to investigate the effects of water-ageing for 6 months on dentine bond strength and anti-biofilm properties for the first time.<br />Methods: Four bonding agents were tested: Scotchbond Multi-Purpose (SBMP) Primer and Adhesive control; SBMP+5% DMADDM; SBMP+5% DMADDM+0.1% NAg; and SBMP+5% DMADDM+0.1% NAg with 20% NACP in adhesive. Specimens were water-aged for 1d and 6 months at 37°C. Then the dentine shear bond strengths were measured. A dental plaque microcosm biofilm model was used to inoculate bacteria on water-aged specimens and to measure metabolic activity, colony-forming units (CFUs), and lactic acid production.<br />Results: Dentine bond strength showed a 35% loss in 6 months of water-ageing for SBMP control (mean±sd; n=10); in contrast, the new antibacterial bonding agents showed no strength loss. The DMADDM-NAg-NACP containing bonding agent imparted a strong antibacterial effect by greatly reducing biofilm viability, metabolic activity and acid production. The biofilm CFU was reduced by more than two orders of magnitude, compared to SBMP control. Furthermore, the DMADDM-NAg-NACP bonding agent exhibited a long-term antibacterial performance, with no significant difference between 1d and 6 months (p>0.1).<br />Conclusions: Incorporating DMADDM-NAg-NACP in bonding agent yielded potent and long-lasting antibacterial properties, and much stronger bond strength after 6 months of water-ageing than a commercial control. The new antibacterial bonding agent is promising to inhibit biofilms and caries at the margins. The method of DMADDM-NAg-NACP incorporation may have a wide applicability to other adhesives, cements and composites.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adult
Anti-Bacterial Agents pharmacology
Bacteria drug effects
Bacterial Load drug effects
Calcium Phosphates chemistry
Calcium Phosphates pharmacology
Dental Plaque microbiology
Dentin-Bonding Agents pharmacology
Humans
Lactic Acid metabolism
Materials Testing
Metal Nanoparticles chemistry
Methacrylates pharmacology
Microbial Viability drug effects
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Nanoparticles chemistry
Quaternary Ammonium Compounds pharmacology
Resin Cements chemistry
Resin Cements pharmacology
Saliva microbiology
Silver chemistry
Silver pharmacology
Stress, Mechanical
Surface Properties
Temperature
Time Factors
Anti-Bacterial Agents chemistry
Biofilms drug effects
Dental Bonding
Dentin ultrastructure
Dentin-Bonding Agents chemistry
Methacrylates chemistry
Quaternary Ammonium Compounds chemistry
Water chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-176X
- Volume :
- 41
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of dentistry
- Publication Type :
- Academic Journal
- Accession number :
- 23583528
- Full Text :
- https://doi.org/10.1016/j.jdent.2013.03.011