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Orthotopic and heterotopic tracheal transplantation model in studying obliterative bronchiolitis.
- Source :
-
Transplant immunology [Transpl Immunol] 2013 Jun; Vol. 28 (4), pp. 170-5. Date of Electronic Publication: 2013 Apr 22. - Publication Year :
- 2013
-
Abstract
- Background: Several animal models have been established to investigate the mechanisms of obliterative bronchiolitis after lung transplantation. In this study, we compared three prevalent murine models of obliterative bronchiolitis in terms of several basic pathologic changes in a relatively short span of time after transplantation.<br />Methods: Each of the recipient mice simultaneously received orthotopic, intra-omental and subcutaneous tracheal transplantation in both syngeneic and allogeneic settings. No immunosuppressive treatment was administered. Tracheal grafts were harvested on Day 14, 21 and 28 after transplantation for histological and immunohistochemical analyses.<br />Results: Syngeneic tracheal grafts from different transplant sites retained normal histologic structures, while their corresponding allografts demonstrated more occlusion of the airway lumen as well as more infiltration of CD4(+)/CD8(+) mononuclear cells and myofibroblasts, but less regenerative epithelium and neovascularized vessels at indicated times (P<0.05). Compared with two heterotopic allografts, orthotopic allografts had less occlusion of the tracheal lumen as well as less infiltration of CD4(+)/CD8(+) mononuclear cells and myofibroblasts, but more regenerative epithelium and neovascularized vessels (P<0.05).<br />Conclusions: Orthotopic tracheal transplantation in mice can be considered as a model to study early stages of obliterative bronchiolitis, and heterotopic tracheal transplantation can be a model for late stages of obliterative bronchiolitis.<br /> (Copyright © 2013 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Bronchiolitis Obliterans immunology
CD4-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes immunology
Female
Lung Transplantation adverse effects
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Models, Animal
Neovascularization, Physiologic immunology
Transplantation, Heterotopic adverse effects
Transplantation, Homologous adverse effects
Bronchiolitis Obliterans pathology
Trachea pathology
Trachea transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5492
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Transplant immunology
- Publication Type :
- Academic Journal
- Accession number :
- 23619376
- Full Text :
- https://doi.org/10.1016/j.trim.2013.04.006