Back to Search
Start Over
Inactivation of the Pta-AckA pathway causes cell death in Staphylococcus aureus.
- Source :
-
Journal of bacteriology [J Bacteriol] 2013 Jul; Vol. 195 (13), pp. 3035-44. Date of Electronic Publication: 2013 Apr 26. - Publication Year :
- 2013
-
Abstract
- During growth under conditions of glucose and oxygen excess, Staphylococcus aureus predominantly accumulates acetate in the culture medium, suggesting that the phosphotransacetylase-acetate kinase (Pta-AckA) pathway plays a crucial role in bacterial fitness. Previous studies demonstrated that these conditions also induce the S. aureus CidR regulon involved in the control of cell death. Interestingly, the CidR regulon is comprised of only two operons, both encoding pyruvate catabolic enzymes, suggesting an intimate relationship between pyruvate metabolism and cell death. To examine this relationship, we introduced ackA and pta mutations in S. aureus and tested their effects on bacterial growth, carbon and energy metabolism, cid expression, and cell death. Inactivation of the Pta-AckA pathway showed a drastic inhibitory effect on growth and caused accumulation of dead cells in both pta and ackA mutants. Surprisingly, inactivation of the Pta-AckA pathway did not lead to a decrease in the energy status of bacteria, as the intracellular concentrations of ATP, NAD(+), and NADH were higher in the mutants. However, inactivation of this pathway increased the rate of glucose consumption, led to a metabolic block at the pyruvate node, and enhanced carbon flux through both glycolysis and the tricarboxylic acid (TCA) cycle. Intriguingly, disruption of the Pta-AckA pathway also induced the CidR regulon, suggesting that activation of alternative pyruvate catabolic pathways could be an important survival strategy for the mutants. Collectively, the results of this study demonstrate the indispensable role of the Pta-AckA pathway in S. aureus for maintaining energy and metabolic homeostasis during overflow metabolism.
- Subjects :
- Bacterial Proteins genetics
Bacterial Proteins metabolism
Citric Acid Cycle genetics
Citric Acid Cycle physiology
Glycolysis genetics
Glycolysis physiology
Phosphate Acetyltransferase genetics
Phosphate Acetyltransferase metabolism
Staphylococcus aureus genetics
Staphylococcus aureus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5530
- Volume :
- 195
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 23625849
- Full Text :
- https://doi.org/10.1128/JB.00042-13