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Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 2013 Jul; Vol. 94 (1), pp. 17-24. Date of Electronic Publication: 2013 Apr 29. - Publication Year :
- 2013
-
Abstract
- Eosinophils are generally linked to innate host defense against helminths, as well as the pathologies associated with allergic diseases, such as asthma. Nonetheless, the activities of eosinophils remain poorly understood, which in turn, has prevented detailed definitions of their role(s) in health and disease. Homologous recombination in embryonic stem cells was used to insert a mammalianized Cre recombinase in the ORF encoding Epx. This knock-in strategy overcame previous inefficiencies associated with eosinophil-specific transgenic approaches and led to the development of a knock-in strain of mice (eoCRE), capable of mediating recombination of "floxed" reporter cassettes in >95% of peripheral blood eosinophils. We also showed that this Cre expression was limited exclusively to eosinophil-lineage committed cells with no evidence of Cre-mediated toxicity. The efficiency and specificity of Cre expression in eoCRE mice were demonstrated further in a cross with a knock-in mouse containing a "(flox-stop-flox)" DTA cassette at the ROSA26 locus, generating yet another novel, eosinophil-less strain of mice. The development of eoCRE mice represents a milestone in studies of eosinophil biology, permitting eosinophil-specific gene targeting and overexpression in the mouse as part of next-generation studies attempting to define eosinophil effector functions.
- Subjects :
- Animals
Bone Marrow metabolism
Cell Differentiation
Cell Lineage
Cell Proliferation
Embryonic Stem Cells cytology
Embryonic Stem Cells metabolism
Flow Cytometry
Homologous Recombination
Humans
Mice
Mice, Inbred C57BL
Mice, Transgenic
Eosinophil Peroxidase physiology
Eosinophils enzymology
Integrases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1938-3673
- Volume :
- 94
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 23630390
- Full Text :
- https://doi.org/10.1189/jlb.0213089