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Altered immunity and dendritic cell activity in the periphery of mice after long-term engraftment with bone marrow from ultraviolet-irradiated mice.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2013 Jun 01; Vol. 190 (11), pp. 5471-84. Date of Electronic Publication: 2013 May 01. - Publication Year :
- 2013
-
Abstract
- Alterations to dendritic cell (DC) progenitors in the bone marrow (BM) may contribute to long-lasting systemic immunosuppression (>28 d) following exposure of the skin of mice to erythemal UV radiation (UVR). DCs differentiated in vitro from the BM of mice 3 d after UVR (8 kJ/m(2)) have a reduced capacity to initiate immunity (both skin and airways) when adoptively transferred into naive mice. Studies in IL-10(-/-) mice suggested that UV-induced IL-10 was not significantly involved. To investigate the immune capabilities of peripheral tissue DCs generated in vivo from the BM of UV-irradiated mice, chimeric mice were established. Sixteen weeks after reconstitution, contact hypersensitivity responses were significantly reduced in mice reconstituted with BM from UV-irradiated mice (UV-chimeric). When the dorsal skin of UV-chimeric mice was challenged with innate inflammatory agents, the hypertrophy induced in the draining lymph nodes was minimal and significantly less than that measured in control-chimeric mice challenged with the same inflammatory agent. When DCs were differentiated from the BM of UV-chimeric mice using FLT3 ligand or GM-CSF + IL-4, the cells maintained a reduced priming ability. The diminished responses in UV-chimeric mice were not due to different numerical or proportional reconstitution of BM or the hematopoietic cells in blood, lymph nodes, and skin. Erythemal UVR may imprint a long-lasting epigenetic effect on DC progenitors in the BM and alter the function of their terminally differentiated progeny.
- Subjects :
- Adoptive Transfer
Animals
Bone Marrow Cells cytology
Bone Marrow Cells drug effects
Bone Marrow Cells immunology
Cell Differentiation drug effects
Cell Differentiation immunology
Cell Differentiation radiation effects
Cell Movement immunology
Chimerism radiation effects
Dendritic Cells cytology
Dendritic Cells drug effects
Dermatitis, Contact immunology
Female
Granulocyte-Macrophage Colony-Stimulating Factor pharmacology
Hypertrophy
Immunity, Innate
Interleukin-4 pharmacology
Lymph Nodes immunology
Lymph Nodes pathology
Lymph Nodes radiation effects
Membrane Proteins pharmacology
Mice
T-Lymphocytes immunology
T-Lymphocytes radiation effects
Bone Marrow Transplantation
Dendritic Cells immunology
Dendritic Cells radiation effects
Graft Survival immunology
Ultraviolet Rays
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 190
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 23636055
- Full Text :
- https://doi.org/10.4049/jimmunol.1202786