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Short-term hypoxia/reoxygenation activates the angiogenic pathway in rat caudate putamen.
- Source :
-
Journal of biosciences [J Biosci] 2013 Jun; Vol. 38 (2), pp. 363-71. - Publication Year :
- 2013
-
Abstract
- In response to hypoxia, tissues have to implement numerous mechanisms to enhance oxygen delivery, including the activation of angiogenesis. This work investigates the angiogenic response of the hypoxic caudate putamen after several recovery times. Adult Wistar rats were submitted to acute hypoxia and analysed after 0 h, 24 h and 5 days of reoxygenation. Expression of hypoxia-inducible factor-1 alfa (HIF-1 alpha) and angiogenesis-related genes including vascular endothelial growth factor (VEGF), adrenomedullin (ADM) and transforming growth factor-beta 1 (TGF- beta 1) was determined by both RT-PCR and ELISA. For vessel labelling, lectin location and expression were analysed using histochemical and image processing techniques (fractal dimension). Expression of Hif-1 alpha, Vegf, Adm and Tgf- beta 1 mRNA rose immediately after hypoxia and this increase persisted in some cases after 5 days post-hypoxia. While VEGF and TGF-beta 1 protein levels increased parallel to mRNA expression, ADM remained unaltered. The quantification of the striatal vessel network showed a significant augmentation at 24 h of reoxygenation. These results reveal that not only short-term hypoxia, but also the subsequent reoxygenation period, up-regulate the angiogenic pathway in the rat caudate putamen as a neuroprotective mechanism to hypoxia that seeks to maintain a proper blood supply to the hypoxic tissue, thereby minimizing the adverse effects of oxygen deprivation.
- Subjects :
- Adrenomedullin genetics
Adrenomedullin metabolism
Animals
Cell Hypoxia
Gene Expression
Hypoxia-Inducible Factor 1, alpha Subunit genetics
Hypoxia-Inducible Factor 1, alpha Subunit metabolism
Lectins metabolism
Male
Putamen blood supply
Putamen cytology
Rats
Rats, Wistar
Transcriptional Activation
Transforming Growth Factor beta1 genetics
Transforming Growth Factor beta1 metabolism
Vascular Endothelial Growth Factor A genetics
Vascular Endothelial Growth Factor A metabolism
Neovascularization, Physiologic
Putamen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0973-7138
- Volume :
- 38
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of biosciences
- Publication Type :
- Academic Journal
- Accession number :
- 23660671
- Full Text :
- https://doi.org/10.1007/s12038-013-9327-6