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An in vivo experimental model to determine antigenic variations among infectious bursal disease viruses.
- Source :
-
Avian pathology : journal of the W.V.P.A [Avian Pathol] 2013 Aug; Vol. 42 (4), pp. 309-15. Date of Electronic Publication: 2013 May 13. - Publication Year :
- 2013
-
Abstract
- Infectious bursal disease virus (IBDV) is a double-stranded RNA virus causing infectious bursal disease in chickens. IBDV undergoes antigenic drift, so characterizing the antigenicity of IBDV plays an important role for identification and selection of vaccine candidates. In this study, an in vivo experimental model was developed to differentiate a new antigenic variant of IBDV. To this end, a hyper-immune serum to IBDV E/Del-type virus was generated in specific pathogen-free chickens and a standard volume of the hyper-immune serum was serially diluted and injected in specific pathogen-free birds via intravenous, subcutaneous, or intramuscular routes. The chickens were bled at different time points in order to evaluate the dynamics of virus neutralization titres. Based on the results, chickens were injected with different serum dilutions by the subcutaneous route. Twenty-four hours later, chickens were bled and then challenged with 100 median chicken infectious doses of the E/Del virus and a new IBDV variant. Chickens were euthanized at 7 days post infection and the bursa of Fabricius was removed for microscopic evaluation to determine the bursal lesion score. The determined virus neutralization titre along with the bursal lesion score was used to determine the breakthrough titre in the in vivo chicken model. Based on the data obtained, an antigenic subtype of IBDV was identified and determined to be different from E/Del. This model is a sensitive model for determination of IBDV antigenicity of non-tissue culture adapted IBDV.
- Subjects :
- Animals
Bursa of Fabricius pathology
Chick Embryo
Immune Sera immunology
Neutralization Tests veterinary
Specific Pathogen-Free Organisms
Antigens, Viral genetics
Birnaviridae Infections veterinary
Chickens
Disease Models, Animal
Infectious bursal disease virus immunology
Poultry Diseases virology
Viral Vaccines genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1465-3338
- Volume :
- 42
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Avian pathology : journal of the W.V.P.A
- Publication Type :
- Academic Journal
- Accession number :
- 23662946
- Full Text :
- https://doi.org/10.1080/03079457.2013.793783