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Ectopic expression of a wheat WRKY transcription factor gene TaWRKY71-1 results in hyponastic leaves in Arabidopsis thaliana.
- Source :
-
PloS one [PLoS One] 2013 May 09; Vol. 8 (5), pp. e63033. Date of Electronic Publication: 2013 May 09 (Print Publication: 2013). - Publication Year :
- 2013
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Abstract
- Leaf type is an important trait that closely associates with crop yield. WRKY transcription factors exert diverse regulatory effects in plants, but their roles in the determination of leaf type have not been reported so far. In this work, we isolated a WRKY transcription factor gene TaWRKY71-1 from a wheat introgression line SR3, which has larger leaves, superior growth capacity and higher yield than its parent common wheat JN177. TaWRKY71-1 specifically expressed in leaves, and produced more mRNA in SR3 than in JN177. TaWRKY71-1 localized in the nucleus and had no transcriptional activation activity. TaWRKY71-1 overexpression in Arabidopsis resulted in hyponastic rosette leaves, and the hyponastic strength was closely correlative with the transcription level of the transgene. The spongy mesophyll cells at abaxial side of leaves were drastically compacted by TaWRKY71-1 overexpression. In TaWRKY71-1 overexpression Arabidopsis, the expression of IAMT1 that encodes a methyltransferase converting free indole-3-acetic acid (IAA) to methyl-IAA ester (MeIAA) to alter auxin homeostatic level was induced, and the induction level was dependent on the abundance of TaWRKY71-1 transcripts. Besides, several TCP genes that had found to be restricted by IAMT1 had lower expression levels as well. Our results suggest that TaWRKY71-1 causes hyponastic leaves through altering auxin homeostatic level by promoting the conversion of IAA to MeIAA.
- Subjects :
- Amino Acid Sequence
Arabidopsis growth & development
Arabidopsis metabolism
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Gene Expression Regulation, Plant
Indoleacetic Acids metabolism
Methyltransferases genetics
Methyltransferases metabolism
Molecular Sequence Data
Phylogeny
Plant Leaves growth & development
Plant Leaves metabolism
Plant Proteins classification
Plant Proteins metabolism
Plants, Genetically Modified
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Species Specificity
Transcription Factors classification
Transcription Factors metabolism
Triticum classification
Arabidopsis genetics
Plant Leaves genetics
Plant Proteins genetics
Transcription Factors genetics
Triticum genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23671653
- Full Text :
- https://doi.org/10.1371/journal.pone.0063033