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Body fluid and tissue analysis using filter paper sampling support prior to LC-MS/MS: application to fatal overdose with colchicine.
- Source :
-
Drug testing and analysis [Drug Test Anal] 2013 Sep-Oct; Vol. 5 (9-10), pp. 763-72. Date of Electronic Publication: 2013 May 16. - Publication Year :
- 2013
-
Abstract
- Because of the various matrices available for forensic investigations, the development of versatile analytical approaches allowing the simultaneous determination of drugs is challenging. The aim of this work was to assess a liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform allowing the rapid quantification of colchicine in body fluids and tissues collected in the context of a fatal overdose. For this purpose, filter paper was used as a sampling support and was associated with an automated 96-well plate extraction performed by the LC autosampler itself. The developed method features a 7-min total run time including automated filter paper extraction (2 min) and chromatographic separation (5 min). The sample preparation was reduced to a minimum regardless of the matrix analyzed. This platform was fully validated for dried blood spots (DBS) in the toxic concentration range of colchicine. The DBS calibration curve was applied successfully to quantification in all other matrices (body fluids and tissues) except for bile, where an excessive matrix effect was found. The distribution of colchicine for a fatal overdose case was reported as follows: peripheral blood, 29 ng/ml; urine, 94 ng/ml; vitreous humour and cerebrospinal fluid, < 5 ng/ml; pericardial fluid, 14 ng/ml; brain, < 5 pg/mg; heart, 121 pg/mg; kidney, 245 pg/mg; and liver, 143 pg/mg. Although filter paper is usually employed for DBS, we report here the extension of this alternative sampling support to the analysis of other body fluids and tissues. The developed platform represents a rapid and versatile approach for drug determination in multiple forensic media.<br /> (Copyright © 2013 John Wiley & Sons, Ltd.)
- Subjects :
- Adult
Calibration
Chromatography, Liquid methods
Colchicine blood
Colchicine cerebrospinal fluid
Dried Blood Spot Testing methods
Filtration instrumentation
Humans
Male
Paper
Sensitivity and Specificity
Specimen Handling methods
Tubulin Modulators blood
Tubulin Modulators cerebrospinal fluid
Body Fluids chemistry
Colchicine analysis
Colchicine poisoning
Tandem Mass Spectrometry methods
Tubulin Modulators analysis
Tubulin Modulators poisoning
Subjects
Details
- Language :
- English
- ISSN :
- 1942-7611
- Volume :
- 5
- Issue :
- 9-10
- Database :
- MEDLINE
- Journal :
- Drug testing and analysis
- Publication Type :
- Academic Journal
- Accession number :
- 23682018
- Full Text :
- https://doi.org/10.1002/dta.1496