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RNA interference-mediated silencing of NANOG leads to reduced proliferation and self-renewal, cell cycle arrest and apoptosis in T-cell acute lymphoblastic leukemia cells via the p53 signaling pathway.
- Source :
-
Leukemia research [Leuk Res] 2013 Sep; Vol. 37 (9), pp. 1170-7. Date of Electronic Publication: 2013 May 14. - Publication Year :
- 2013
-
Abstract
- NANOG is critical for maintaining the self-renewal and proliferative properties of embryonic stem cells. Here we found that cultured T-cell acute lymphoblastic leukemia (T-ALL) cells, as well as human primary T-ALL cells, express a functional variant of NANOG. NANOG mRNA is derived predominantly from a retrogene locus termed NANOGP8. Furthermore, we showed that RNA interference-mediated NANOG knockdown inhibited cell proliferation, reduced self-renewal, promoted apoptosis and arrested the cell cycle through a p53-mediated pathway in leukemic cells. These findings demonstrate the oncogenic potential of this pluripotent gene in human T-ALL cells.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Biomarkers, Tumor genetics
Biomarkers, Tumor metabolism
Blotting, Western
Cell Differentiation
Colony-Forming Units Assay
Gene Expression Profiling
Homeodomain Proteins antagonists & inhibitors
Homeodomain Proteins genetics
Humans
Nanog Homeobox Protein
Oligonucleotide Array Sequence Analysis
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma genetics
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma metabolism
RNA, Messenger genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Tumor Suppressor Protein p53 genetics
Apoptosis
Cell Cycle Checkpoints
Cell Proliferation
Homeodomain Proteins metabolism
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma pathology
RNA Interference
Tumor Suppressor Protein p53 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5835
- Volume :
- 37
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Leukemia research
- Publication Type :
- Academic Journal
- Accession number :
- 23683786
- Full Text :
- https://doi.org/10.1016/j.leukres.2013.04.021