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Restoration of sensitivity in chemo-resistant glioma cells by cold atmospheric plasma.
- Source :
-
PloS one [PLoS One] 2013 May 21; Vol. 8 (5), pp. e64498. Date of Electronic Publication: 2013 May 21 (Print Publication: 2013). - Publication Year :
- 2013
-
Abstract
- Glioblastoma (GBM) is the most common and aggressive brain tumor in adults. Despite multimodal treatments including surgery, chemotherapy and radiotherapy the prognosis remains poor and relapse occurs regularly. The alkylating agent temozolomide (TMZ) has been shown to improve the overall survival in patients with malignant gliomas, especially in tumors with methylated promoter of the O6-methylguanine-DNA-methyltransferase (MGMT) gene. However, intrinsic and acquired resistance towards TMZ makes it crucial to find new therapeutic strategies aimed at improving the prognosis of patients suffering from malignant gliomas. Cold atmospheric plasma is a new auspicious candidate in cancer treatment. In the present study we demonstrate the anti-cancer properties of different dosages of cold atmospheric plasma (CAP) both in TMZ-sensitive and TMZ-resistant cells by proliferation assay, immunoblotting, cell cycle analysis, and clonogenicity assay. Importantly, CAP treatment restored the responsiveness of resistant glioma cells towards TMZ therapy. Concomitant treatment with CAP and TMZ led to inhibition of cell growth and cell cycle arrest, thus CAP might be a promising candidate for combination therapy especially for patients suffering from GBMs showing an unfavorable MGMT status and TMZ resistance.
- Subjects :
- Adult
Brain Neoplasms pathology
Cell Death drug effects
Cell Division drug effects
Cell Line, Tumor
Cell Proliferation drug effects
DNA Damage
Dacarbazine analogs & derivatives
Dacarbazine pharmacology
Dacarbazine therapeutic use
Dose-Response Relationship, Drug
G2 Phase drug effects
Glioma pathology
Humans
Plasma Gases pharmacology
Temozolomide
Tumor Stem Cell Assay
Atmosphere
Brain Neoplasms drug therapy
Drug Resistance, Neoplasm drug effects
Glioma drug therapy
Plasma Gases therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23704990
- Full Text :
- https://doi.org/10.1371/journal.pone.0064498