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Development of a chimeric Plasmodium berghei strain expressing the repeat region of the P. vivax circumsporozoite protein for in vivo evaluation of vaccine efficacy.
- Source :
-
Infection and immunity [Infect Immun] 2013 Aug; Vol. 81 (8), pp. 2882-7. Date of Electronic Publication: 2013 May 28. - Publication Year :
- 2013
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Abstract
- The development of vaccine candidates against Plasmodium vivax-the most geographically widespread human malaria species-is challenged by technical difficulties, such as the lack of in vitro culture systems and availability of animal models. Chimeric rodent Plasmodium parasites are safe and useful tools for the preclinical evaluation of new vaccine formulations. We report the successful development and characterization of chimeric Plasmodium berghei parasites bearing the type I repeat region of P. vivax circumsporozoite protein (CSP). The P. berghei-P. vivax chimeric strain develops normally in mosquitoes and produces highly infectious sporozoites that produce patent infection in mice that are exposed to the bites of as few as 3 P. berghei-P. vivax-infected mosquitoes. Using this transgenic parasite, we demonstrate that monoclonal and polyclonal antibodies against P. vivax CSP strongly inhibit parasite infection and thus support the notion that these antibodies play an important role in protective immunity. The chimeric parasites we developed represent a robust model for evaluating protective immune responses against P. vivax vaccines based on CSP.
- Subjects :
- Animals
Antibodies, Protozoan immunology
Antigens, Protozoan immunology
Chimera immunology
Fluorescent Antibody Technique, Indirect
Mice
Plasmodium berghei immunology
Plasmodium vivax immunology
Protozoan Proteins immunology
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Chimera genetics
Malaria Vaccines immunology
Plasmodium berghei genetics
Plasmodium vivax genetics
Protozoan Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5522
- Volume :
- 81
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 23716612
- Full Text :
- https://doi.org/10.1128/IAI.00461-13