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Deletion of virulence associated genes from attenuated African swine fever virus isolate OUR T88/3 decreases its ability to protect against challenge with virulent virus.

Authors :
Abrams CC
Goatley L
Fishbourne E
Chapman D
Cooke L
Oura CA
Netherton CL
Takamatsu HH
Dixon LK
Source :
Virology [Virology] 2013 Aug 15; Vol. 443 (1), pp. 99-105. Date of Electronic Publication: 2013 May 28.
Publication Year :
2013

Abstract

African swine fever virus (ASFV) causes an acute haemorrhagic disease of domestic pigs against which there is no effective vaccine. The attenuated ASFV strain OUR T88/3 has been shown previously to protect vaccinated pigs against challenge with some virulent strains including OUR T88/1. Two genes, DP71L and DP96R were deleted from the OUR T88/3 genome to create recombinant virus OUR T88/3ΔDP2. Deletion of these genes from virulent viruses has previously been shown to reduce ASFV virulence in domestic pigs. Groups of 6 pigs were immunised with deletion virus OUR T88/3ΔDP2 or parental virus OUR T88/3 and challenged with virulent OUR T88/1 virus. Four pigs (66%) were protected by inoculation with the deletion virus OUR T88/3ΔDP2 compared to 100% protection with the parental virus OUR T88/3. Thus the deletion of the two genes DP71L and DP96R from OUR T88/3 strain reduced its ability to protect pigs against challenge with virulent virus.<br /> (Crown Copyright © 2013. Published by Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1096-0341
Volume :
443
Issue :
1
Database :
MEDLINE
Journal :
Virology
Publication Type :
Academic Journal
Accession number :
23725691
Full Text :
https://doi.org/10.1016/j.virol.2013.04.028