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A reliable method to quantify sildenafil and its metabolite N-demethylsildenafil by HPLC in plasma of children.
- Source :
-
Drug research [Drug Res (Stuttg)] 2013 Sep; Vol. 63 (9), pp. 473-6. Date of Electronic Publication: 2013 Jun 04. - Publication Year :
- 2013
-
Abstract
- The aim of this study was to develop a simple method for quantifying plasma levels of sildenafil and its metabolite by liquid chromatography with a C18 reverse-phase column and UV detection. For both compounds, linearity was assessed in the range from 10 and 1 000 ng · ml-1 and had correlation coefficients of r=0.995 and r=0.997 for sildenafil and its metabolite, respectively. The inter- and intra-day coefficients of variation was<5.3%. The limits of detection and quantification were 1 and 10 ng · ml-1. Drug levels were determined satisfactorily in two patients. A simple and reliable method was developed for use in children with Pulmonary Arterial Hypertension under treatment with sildenafil.<br /> (© Georg Thieme Verlag KG Stuttgart · New York.)
Details
- Language :
- English
- ISSN :
- 2194-9379
- Volume :
- 63
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Drug research
- Publication Type :
- Academic Journal
- Accession number :
- 23737285
- Full Text :
- https://doi.org/10.1055/s-0033-1345183